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p63 Antibody / Tumor Protein p63 Antibody

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p63 Antibody / Tumor Protein p63 Antibody

p63 Transcription Factor Function and Epithelial Biology

Tumor protein p63 (TP63), commonly known as p63, is a transcription factor that plays a central role in epithelial development, stem cell maintenance, and squamous cell differentiation. The p63 antibody is widely used to study epithelial lineage and tissue organization, particularly in stratified epithelia where p63 expression is associated with basal cell populations. Its role in regulating epithelial integrity makes it a key target in developmental biology and cancer research.

p63 antibody, also referred to as TP63 antibody or squamous epithelial marker antibody, enables detection of nuclear protein expression in epithelial tissues and tumors. p63 is primarily localized to the nucleus of basal epithelial cells, where it regulates gene expression programs involved in proliferation and differentiation. In epithelial cancers, p63 expression is often evaluated alongside p53 tumor suppressor antibody to better understand tumor biology and differentiation status.

p63 Antibody in Epithelial Differentiation

p63 is essential for the development and maintenance of stratified epithelial tissues. It regulates genes involved in cell adhesion, proliferation, and differentiation, supporting the formation of organized epithelial layers. p63 antibody enables investigation of these processes in both normal tissue development and disease states.

p63 Antibody in Squamous Cell Biology

p63 is strongly associated with squamous cell differentiation and is widely used as a marker of squamous epithelial cells. Its expression is particularly relevant in studies of squamous cell carcinomas and other epithelial malignancies. p63 antibody supports analysis of these tissues by enabling detection of basal and progenitor cell populations.

p63 Antibody in p53 Family Signaling

As a member of the p53 family of transcription factors, p63 shares structural and functional similarities with p53 and p73. However, p63 exhibits distinct expression patterns and biological roles, particularly in epithelial tissues. This relationship makes p63 antibody useful for studying transcriptional regulation and lineage-specific signaling pathways within this protein family.

p63 Antibody in Tissue Development and Regeneration

p63 is involved in tissue regeneration and wound healing, where it contributes to epithelial cell renewal and repair. p63 antibody enables analysis of these functions by allowing detection of protein expression in regenerating tissues and experimental models.

Due to its importance in epithelial biology and cancer classification, p63 remains a widely used marker for studying tissue development, differentiation, and tumor identity.

For additional antibody categories and targets, explore our antibody overview page.

Please find a selection of NSJ Bioreagents p63 antibodies below.

<p><strong data-start="147" data-end="329">p63 Antibody for IHC. Immunohistochemistry analysis of p63 / TP63 expression in human prostate cancer tissue and protein microarray specificity validation using clone TP63/4379R.</strong> FFPE human prostate cancer tissue stained with recombinant p63 antibody shows strong nuclear HRP-DAB brown signal in basal-type epithelial cells, with surrounding stromal cells largely negative, consistent with the known nuclear localization of Tumor protein p63. Antigen retrieval was performed using Tris-EDTA buffer (pH 9) to enhance epitope accessibility. In parallel, protein microarray analysis using a HuProt array containing over 19,000 full-length human proteins demonstrates highly selective binding of clone TP63/4379R to TP63, with a dominant top-ranked signal, high Z-score, and strong separation from non-target proteins. Minimal off-target interaction and clear signal discrimination support the specificity of this p63 antibody for TP63 detection in tissue-based and proteome-wide applications.</p>

p63 Antibody for IHC. Immunohistochemistry analysis of p63 / TP63 expression in human prostate cancer tissue and protein microarray specificity validation using clone TP63/4379R. FFPE human prostate cancer tissue stained with recombinant p63 antibody shows strong nuclear HRP-DAB brown signal in basal-type epithelial cells, with surrounding stromal cells largely negative, consistent with the known nuclear localization of Tumor protein p63. Antigen retrieval was performed using Tris-EDTA buffer (pH 9) to enhance epitope accessibility. In parallel, protein microarray analysis using a HuProt array containing over 19,000 full-length human proteins demonstrates highly selective binding of clone TP63/4379R to TP63, with a dominant top-ranked signal, high Z-score, and strong separation from non-target proteins. Minimal off-target interaction and clear signal discrimination support the specificity of this p63 antibody for TP63 detection in tissue-based and proteome-wide applications.

Found all antibodies, displaying 1 to 10
p63 Antibody / Tumor protein 63 (clone TP63/4379R)
Catalog No : V9353
Applications : IHC-P
Reactivity : Human
Format : Purified
Microvalidated
Recrabbitmono
p63 Antibody Microarray Specificity Validated Clone TP63/4379R. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human prostate cancer tissue using a recombinant rabbit monoclonal p63 antibody for IHC (clone TP63/4379R) demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells, while malignant glandular epithelial cells show little to no staining. This distinct basal-restricted pattern supports the role of TP63 as a basal cell marker and highlights loss of basal cell layer in prostate carcinoma. The clean nuclear signal with minimal background is consistent with the high specificity profile established by microarray validation, supporting accurate interpretation of TP63 expression in tissue sections. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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p63 Antibody / Tumor protein 63 (clone TMPR63-1R)
Catalog No : V3816
Applications : IHC-P
Reactivity : Human
Format : Purified
Recrabbitmono
Immunohistochemistry analysis of p63 expression. Recombinant TP63 antibody (clone TMPR63-1R) staining was performed on formalin-fixed, paraffin-embedded human prostate cancer tissue, showing nuclear DAB-positive staining in basal-type epithelial cells lining glandular structures, with surrounding stromal cells largely negative and hematoxylin counterstaining. Heat-induced epitope retrieval was carried out by boiling tissue sections in Tris buffer (10 mM, pH 9.0) containing EDTA (1 mM) for 10-20 minutes, followed by cooling at room temperature for 20 minutes, with signal detection using an HRP-conjugated secondary antibody and DAB chromogen.
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p63 Antibody / Tumor protein 63 (clone RM383)
Catalog No : R20340
Applications : WB, IHC-P
Reactivity : Human
Format : Purified
Recrabbitmono
p63 Antibody. Western blot analysis of human A431 cell lysate using a p63 antibody as a basal cell carcinoma marker demonstrates detection of Tumor protein 63 (TP63) as a band at the predicted molecular weight of approximately 63-77 kDa. The band corresponds to TP63 isoforms commonly expressed in basal epithelial-derived cells, consistent with the known biology of A431 cells as a squamous carcinoma line. The signal appears as a distinct band with low background, supporting specific detection of TP63 in denatured lysates. This result aligns with the role of TP63 as a basal cell-associated transcription factor and supports the use of this p63 antibody for western blot analysis of basal-like tumor cell populations.
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p63 Antibody / Tumor protein 63 (clone TP63/7807R)
Catalog No : V5027
Applications : IHC-P
Reactivity : Human
Format : Purified
Recrabbitmono
TP63 Antibody. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human skin tissue using a TP63 antibody as an epithelial stem cell marker (clone TP63/7807R) demonstrates strong HRP-DAB brown nuclear staining in basal keratinocytes along the epidermal basal layer. The staining is confined to progenitor cell populations at the dermal-epidermal junction, while suprabasal differentiated keratinocytes show reduced or absent signal, consistent with the role of TP63 in maintaining epithelial stem and progenitor cell compartments. The nuclear localization clearly outlines the basal layer and highlights epidermal organization and stratification. Inset: PBS was used in place of the primary antibody as a negative control, showing no brown signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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p63 Antibody / Tumor protein 63 (clone TP63/1786)
Catalog No : V3368
Applications : ELISA, WB
Reactivity : Human
Format : Purified
Microvalidated
p63 Antibody for WB. Western blot analysis of human MCF-7, T-47D, and MDA-MB-453 cell lysates using mouse monoclonal clone TP63/1786 demonstrates detection of Tumor protein 63 (TP63) as a band at the predicted molecular weight of approximately 63-77 kDa. Signal intensity varies across the cell lines, with stronger expression observed in MDA-MB-453 cells and comparatively lower levels in MCF-7 and T-47D, consistent with differences in TP63 expression associated with epithelial subtype characteristics. The clean banding pattern with minimal background supports the specificity of clone TP63/1786, aligning with its microarray-validated target recognition and suitability for western blot detection of TP63 isoforms.
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p63 Antibody for IF / Tumor protein 63
Catalog No : R31572
Applications : WB, IHC-P, IF, FACS
Reactivity : Human
Format : Antigen affinity purified
p63 Antibody for IF. Immunofluorescence analysis of FFPE human prostate hyperplasia tissue using a rabbit polyclonal p63 antibody for IF demonstrates strong nuclear staining in basal epithelial cells (red), consistent with the role of Tumor protein 63 (TP63) as a nuclear transcription factor. Signal is restricted to the basal cell layer surrounding glandular structures, while luminal epithelial cells remain largely negative, clearly outlining epithelial architecture and basal cell compartments. DAPI nuclear counterstain (blue) labels all nuclei, providing contrast for precise identification of TP63-positive cells within the tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to antibody incubation.
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p63 Antibody / Tumor protein 63
Catalog No : R31167
Applications : WB, IHC-P, ICC, IF, FACS
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human A431 cells with p63 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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p63 Antibody / Tumor protein 63 (clone TP63/11)
Catalog No : V7466
Applications : IHC-P
Reactivity : Human, Mouse
Format : Purified
p63 Antibody Clone TP63/11. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human prostate cancer tissue using a mouse monoclonal p63 antibody (clone TP63/11) demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells surrounding glandular structures, while malignant epithelial cells within the glands remain negative. This basal-restricted nuclear staining pattern is consistent with the role of TP63 as a basal cell marker and highlights the absence of a basal cell layer in prostate carcinoma. The crisp nuclear signal with low background supports clear interpretation of epithelial architecture and cell lineage. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.
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