p63 Antibody for IF / TP63 Immunofluorescence Antibody (R31572)

p63 Antibody for IF. Immunofluorescence analysis of FFPE human prostate hyperplasia tissue using a rabbit polyclonal p63 antibody for IF demonstrates strong nuclear staining in basal epithelial cells (red), consistent with the role of Tumor protein 63 (TP63) as a nuclear transcription factor. Signal is restricted to the basal cell layer surrounding glandular structures, while luminal epithelial cells remain largely negative, clearly outlining epithelial architecture and basal cell compartments. DAPI nuclear counterstain (blue) labels all nuclei, providing contrast for precise identification of TP63-positive cells within the tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to antibody incubation.

p63 Antibody for IF. Immunofluorescence analysis of FFPE human A431 cells using a rabbit polyclonal p63 antibody for IF demonstrates strong nuclear staining in TP63-positive cells (green), consistent with the role of Tumor protein 63 as a nuclear transcription factor. Signal is confined to the nucleus with minimal cytoplasmic background, allowing clear identification of TP63-expressing cells within the population. DAPI nuclear counterstain (blue) labels all nuclei, enabling direct comparison between positive and negative cells and highlighting cell-to-cell variability in TP63 expression. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to antibody incubation.

IHC staining of FFPE human esophagus squama cancer tissue with p63 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human A431 cell lysate with p63 antibody. Predicted molecular weight: 47-77 kDa (multiple isoforms).

Flow cytometry testing of fixed and permeabilized human A431 cells with p63 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p63 antibody.