p63 Antibody Microarray Specificity Validated / TP63 HuProt-Validated Antibody [clone TP63/4379R] (V9353)

p63 Antibody Microarray Specificity Validated Clone TP63/4379R. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human prostate cancer tissue using a recombinant rabbit monoclonal p63 antibody for IHC (clone TP63/4379R) demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells, while malignant glandular epithelial cells show little to no staining. This distinct basal-restricted pattern supports the role of TP63 as a basal cell marker and highlights loss of basal cell layer in prostate carcinoma. The clean nuclear signal with minimal background is consistent with the high specificity profile established by microarray validation, supporting accurate interpretation of TP63 expression in tissue sections. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human prostate cancer tissue with recombinant p63 antibody (clone TP63/4379R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate cancer tissue with recombinant p63 antibody (clone TP63/4379R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

p63 Antibody Microarray Specificity Validated Clone TP63/4379R. Protein microarray analysis using a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding of recombinant rabbit monoclonal clone TP63/4379R to Tumor protein 63 (TP63). The antibody shows a dominant top-ranked signal for TP63 with a very high Z-score and strong separation from all other proteins, indicating robust target recognition with minimal off-target interaction. Signals for non-target proteins are negligible, supporting a clean specificity profile across the proteome. Z-score reflects signal intensity relative to the array mean, while S-score represents the separation between the TP63 signal and the next highest-ranking proteins, confirming clear discrimination of the intended target. These results support the use of this p63 antibody for applications requiring high specificity and confident interpretation of TP63 detection.