p63 Antibody for FACS / TP63 Flow Cytometry Antibody (R31167)

Immunofluorescent staining of FFPE human A431 cells with p63 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC-P: p63 antibody testing of human esophageal squamous cell carcinoma tissue

IHC-P: p63 antibody testing of human esophageal squamous cell carcinoma tissue

IHC-P: p63 antibody testing of human lung cancer tissue

ICC testing of human A549 cells with p63 antibody.

Western blot testing of 1) rat heart and 2) mouse thymus lysate (50ug/lane) with p63 antibody. Expected molecular weight: 63-77 kDa.

Western blot testing of human 1) HeLa, 2) SMMC-7721, 3) COLO-320, 4) A549 and 5) SGC-7901 cell lysate with p63 antibody. Expected molecular weight: 63-77 kDa.

p63 Antibody for FACS. Flow cytometry analysis of human A431 cells using a rabbit polyclonal p63 antibody for FACS demonstrates a clear rightward fluorescence shift (blue) relative to isotype control (green) and unstained cells (red), consistent with intracellular detection of Tumor protein 63 (TP63). Cells were fixed and permeabilized to allow access to the nuclear target, resulting in distinct separation of TP63-positive signal from background. The tight peak distribution indicates uniform nuclear expression within the cell population, supporting reliable gating of TP63-expressing cells. Staining was performed at 1 ug per million cells with goat sera blocking to reduce non-specific binding, enabling accurate assessment of TP63 expression by flow cytometry.