p63 Antibody / Basal Cell Carcinoma Marker Antibody [clone RM383] (R20340)

p63 Antibody. Western blot analysis of human A431 cell lysate using a p63 antibody as a basal cell carcinoma marker demonstrates detection of Tumor protein 63 (TP63) as a band at the predicted molecular weight of approximately 63-77 kDa. The band corresponds to TP63 isoforms commonly expressed in basal epithelial-derived cells, consistent with the known biology of A431 cells as a squamous carcinoma line. The signal appears as a distinct band with low background, supporting specific detection of TP63 in denatured lysates. This result aligns with the role of TP63 as a basal cell-associated transcription factor and supports the use of this p63 antibody for western blot analysis of basal-like tumor cell populations.

p63 Antibody. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human tonsil tissue using a p63 antibody as a basal cell carcinoma marker demonstrates strong HRP-DAB brown nuclear staining in basal and parabasal epithelial cells within the stratified squamous epithelium. The staining is confined to nuclei of epithelial cells lining the tonsillar surface, while underlying lymphoid tissue and stromal components remain largely negative. This pattern reflects the basal cell-associated expression of TP63 and highlights epithelial compartmentalization within tonsil tissue. The crisp nuclear localization and clear contrast between epithelial and non-epithelial regions support reliable interpretation of TP63-positive cell populations. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6 citrate buffer for 10-20 minutes followed by cooling prior to antibody incubation.

p63 Antibody. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human breast tissue using a p63 antibody as a basal cell carcinoma marker demonstrates strong HRP-DAB brown nuclear staining in myoepithelial cells surrounding glandular and ductal structures. Luminal epithelial cells within ducts remain largely negative, producing a clear basal-restricted pattern that outlines ductal architecture. This nuclear staining pattern reflects the role of TP63 in basal and myoepithelial cell populations and supports its use in identifying basal lineage cells within breast tissue. The clear contrast between TP63-positive myoepithelial cells and negative luminal cells enables confident interpretation of epithelial organization. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6 citrate buffer for 10-20 minutes followed by cooling prior to antibody incubation.

IHC testing of FFPE human prostate tissue with recombinant p63 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.