p63 Antibody for WB / TP63 Western Blot Antibody [clone TP63/1786] (V3368)

p63 Antibody for WB. Western blot analysis of human MCF-7, T-47D, and MDA-MB-453 cell lysates using mouse monoclonal clone TP63/1786 demonstrates detection of Tumor protein 63 (TP63) as a band at the predicted molecular weight of approximately 63-77 kDa. Signal intensity varies across the cell lines, with stronger expression observed in MDA-MB-453 cells and comparatively lower levels in MCF-7 and T-47D, consistent with differences in TP63 expression associated with epithelial subtype characteristics. The clean banding pattern with minimal background supports the specificity of clone TP63/1786, aligning with its microarray-validated target recognition and suitability for western blot detection of TP63 isoforms.

p63 Antibody for WB protein microarray specificity validation using a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding of mouse monoclonal clone TP63/1786 to Tumor protein 63 (TP63). The antibody shows a dominant top-ranked signal for TP63 with a markedly elevated Z-score compared to all other proteins on the array, indicating strong and specific target recognition. Minimal signal is observed for non-target proteins, supporting low off-target binding and high analytical specificity. Z-score reflects signal intensity relative to the array mean, while S-score represents the separation between the top-ranked target and subsequent proteins, confirming clear discrimination of TP63 from other candidates. These results support the suitability of clone TP63/1786 for western blot applications requiring high specificity and clean band interpretation.

SDS-PAGE analysis of purified, BSA-free p63 antibody (clone TP63/1786) as confirmation of integrity and purity.