p63 Antibody Clone TP63/11 / TP63 Monoclonal Antibody [clone TP63/11] (V7466)

p63 Antibody Clone TP63/11. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human prostate cancer tissue using a mouse monoclonal p63 antibody (clone TP63/11) demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells surrounding glandular structures, while malignant epithelial cells within the glands remain negative. This basal-restricted nuclear staining pattern is consistent with the role of TP63 as a basal cell marker and highlights the absence of a basal cell layer in prostate carcinoma. The crisp nuclear signal with low background supports clear interpretation of epithelial architecture and cell lineage. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

p63 Antibody Clone TP63/11. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE mouse prostate tissue using a mouse monoclonal p63 antibody (clone TP63/11) demonstrates distinct HRP-DAB brown nuclear staining in basal epithelial cells lining prostatic glands. Luminal epithelial cells show minimal to no staining, producing a clear basal-restricted pattern that outlines glandular architecture and epithelial organization. The nuclear localization and cell-type specificity are consistent with TP63 function as a basal cell marker. Background staining is low, allowing confident identification of TP63-positive cells within the tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free p63 antibody (clone TP63/11) as confirmation of integrity and purity.