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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
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Signal Transduction Antibodies

Home >> Antibodies >> Signal Transduction Antibodies

Signal Transduction Antibodies

NSJ Bioreagents offers the following antibodies in the area of Signal Transduction:
Found all antibodies, displaying 11031 to 11040
p53 Antibody / TP53 (clone rTP53/3780)
Catalog No : V6013
Applications : FACS, WB
Reactivity : Human
Format : Purified
Recmousemono
TP53 Antibody for Flow Cytometry / Cellular Tumor Antigen p53 FACS Antibody. Flow cytometry analysis of human HeLa cells stained with TP53 antibody clone rTP53/3780 following paraformaldehyde fixation shows a clear rightward fluorescence shift of the antibody-stained population (red histogram) compared with the isotype control (blue histogram). The shift indicates specific intracellular detection of Tumor protein p53 / TP53 in HeLa cells. The fluorescence distribution reveals a distinct positive population with minimal overlap with the control, consistent with nuclear accumulation of p53 in this carcinoma cell line. The x-axis represents FITC-A fluorescence intensity on a logarithmic scale and the y-axis represents events normalized to mode.
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p53 Antibody / TP53 (clone rIMX25)
Catalog No : V6014
Applications : IHC, WB
Reactivity : Human, Mouse
Format : Purified
Recmousemono
Western blot analysis of Tumor Suppressor p53 / TP53 antibody in human A431 cell lysate (clone rTP53/13508). A distinct band is observed at approximately 53 kDa, consistent with the predicted molecular weight of Tumor protein 53. The detected band corresponds to endogenous p53 expressed in A431 cells. The migration pattern aligns with the expected size of full length TP53 under reducing SDS-PAGE conditions.
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Ubiquitin Antibody (clone rFPM1)
Catalog No : V6015
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
Immunohistochemistry analysis of UBB/Ubiquitin antibody in human brain tissue (clone rFPM1). FFPE human brain sections demonstrate scattered HRP-DAB brown staining within neuronal cell bodies and glial cells, with a predominantly cytoplasmic punctate pattern and minimal nuclear signal. The staining appears granular and localized within the cytoplasm of scattered neural cells against a lightly stained neuropil background, consistent with ubiquitinated protein aggregates and intracellular ubiquitin pools. Surrounding parenchyma shows low diffuse background staining. The inset image shows the PBS secondary-only negative control, confirming absence of specific staining in the control section. Heat induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95C followed by cooling at room temperature prior to primary antibody incubation.
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NANOG Antibody / Homeobox protein NANOG (clone NANOG/9704)
Catalog No : V6024
Applications : IHC
Reactivity : Human
Format : Purified
Immunohistochemistry analysis of NANOG Transcription Factor/Nanog antibody (clone NANOG/9704) in human testis tissue. Formalin-fixed, paraffin-embedded human testis demonstrates predominant nuclear HRP-DAB brown staining in subsets of germ cells within seminiferous tubules, consistent with NANOG expression, while surrounding stromal cells show minimal staining. Nuclear counterstain highlights tissue architecture. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of specific staining. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.
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ABCC3 Antibody / ATP-binding cassette subfamily C member 3 (clone rDTX1)
Catalog No : V6027
Applications : IHC, WB
Reactivity : Human
Format : Purified
Recmousemono
Western blot analysis of ABCC3 / MRP3 antibody (clone rDTX1) in human PANC28 cell lysate. A distinct band is detected at approximately 65-70 kDa, consistent with the predicted molecular weight range of Multidrug resistance-associated protein 3 and its commonly observed migration on SDS-PAGE. The signal supports expression of ABCC3 in PANC28 pancreatic carcinoma cells.
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Cyclin E1 Antibody / CCNE1 (clone r13A3)
Catalog No : V6029
Applications : IHC, WB
Reactivity : Human
Format : Purified
Recmousemono
Immunohistochemistry analysis of CCNE1 / Cyclin E1 antibody (clone r13A3) in human placental tissue. Formalin-fixed, paraffin-embedded placenta demonstrates nuclear HRP-DAB brown staining in scattered trophoblastic cells, consistent with the known nuclear localization of Cyclin E1 in proliferating cells. Most stromal and non-proliferative cells remain negative, while hematoxylin counterstain highlights overall villous architecture and nuclei. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.
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CD19 Antibody / CD19 molecule (clone CD19/4260R)
Catalog No : V6034
Applications : IHC, WB
Reactivity : Human
Format : Purified
Microvalidated
Recrabbitmono
Western blot analysis of CD19/CD19 molecule antibody in human tonsil tissue lysate. A strong band is detected at approximately 95 kDa, consistent with the predicted molecular weight of CD19 (Cluster of Differentiation 19), a glycosylated B cell surface receptor. The observed band migrates slightly above the core protein size, which is expected due to N-linked glycosylation of CD19. Blot was probed with CD19/CD19 molecule antibody (clone CD19/4260R).
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CD19 Antibody / B lymphocyte antigen CD19 (clone CD19/13031R)
Catalog No : V6035
Applications : ELISA
Reactivity : Human
Format : Purified
Recrabbitmono
SDS-PAGE analysis of B lymphocyte antigen CD19 Antibody under reducing (R) and non-reducing (NR) conditions. Purified B lymphocyte antigen CD19 Antibody (clone CD19/13031R) was loaded at 2 ug per lane. Under non-reducing conditions, the antibody migrates as an intact IgG at approximately 150 kDa. Under reducing conditions, distinct bands corresponding to the heavy chain at approximately 50 kDa and light chain at approximately 25 kDa are observed, confirming expected antibody structure, purity, and integrity.
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MUC16 Antibody / CA125 (clone CA125/8894)
Catalog No : V6039
Applications : IHC
Reactivity : Human
Format : Purified
Immunohistochemistry analysis of CA125 Antibody (Clone CA125/8894) in FFPE human ovarian carcinoma tissue. Strong membranous and apical cytoplasmic HRP-DAB brown staining is observed in malignant epithelial cells forming glandular structures, consistent with overexpression of MUC16 / Cancer antigen 125 in ovarian carcinoma, while surrounding stromal elements are largely negative. Clone CA125/8894 demonstrates distinct epithelial surface localization. The inset shows PBS used in place of the primary antibody as a secondary-only negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes.
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COL4A1 Antibody / Collagen type IV alpha 1 chain (clone MSVA-704R)
Catalog No : V6065
Applications : IHC
Reactivity : Human
Format : Purified
Recrabbitmono
COL4A1 Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Collagen type IV alpha 1 chain COL4A1 in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant rabbit monoclonal COL4A1 antibody clone MSVA-704R. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates strong linear localization along basement membrane structures surrounding epithelial glands, vascular endothelium, and stromal interfaces, consistent with extracellular matrix distribution. In normal tissue microarrays, prominent basement membrane staining is observed in kidney, lung, gastrointestinal tract, and skin, while within tumor tissue microarrays, staining outlines preserved or disrupted basement membrane architecture in selected carcinomas. Evaluation across large TMA panels enables direct comparison of COL4A1 expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported COL4A1 expression profiles in the Human Protein Atlas, supporting its use for basement membrane visualization and extracellular matrix studies.
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