PPIG Antibody / Cyclophilin G Antibody (FY13513)

PPIG Antibody by IHC in Human Esophageal Carcinoma Tissue. Immunohistochemistry staining of FFPE human esophageal cancer tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in numerous tumor cell nuclei, with moderate to strong brown staining distributed throughout the malignant cell population. Nuclear labeling is accompanied by weaker cytoplasmic staining in some cells, while surrounding stromal elements exhibit comparatively limited immunoreactivity. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Gastric Carcinoma Glands. Immunohistochemistry staining of FFPE human stomach cancer tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in malignant glandular epithelial cells, with prominent nuclear-associated brown staining and variable cytoplasmic labeling. The tumor glands show relatively strong staining compared with adjacent stromal elements, which exhibit limited immunoreactivity. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Breast Carcinoma Tissue. Immunohistochemistry staining of FFPE human breast cancer tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G expression is demonstrated by brown nuclear-associated staining in a subset of tumor cells, with variable intensity across the tissue section. Some cells exhibit additional diffuse cytoplasmic labeling, while others remain predominantly hematoxylin-positive. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Tonsil Tissue. Immunohistochemistry staining of FFPE human tonsil tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in scattered lymphoid and stromal cells, with weak to moderate nuclear-associated brown staining. The densely populated lymphoid regions contain numerous hematoxylin-counterstained nuclei with comparatively limited DAB labeling. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Pancreatic Carcinoma with Stromal Infiltration. Immunohistochemistry staining of FFPE human pancreatic cancer tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is detected in scattered tumor cells within a prominent stromal background, with variable nuclear-associated and weak cytoplasmic staining. The heterogeneous labeling pattern contrasts with numerous surrounding cells showing little detectable immunoreactivity. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Pancreatic Carcinoma Cell Clusters. Immunohistochemistry staining of a second FFPE human pancreatic cancer section using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in densely arranged tumor cells, with moderate nuclear-associated staining and variable cytoplasmic labeling. The tumor cell clusters exhibit more extensive staining than the surrounding stromal regions. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Placental Chorionic Villi. Immunohistochemistry staining of FFPE human placenta tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in cells lining chorionic villous structures, with predominantly nuclear-associated brown staining and additional diffuse cytoplasmic labeling in some regions. The staining varies among individual villi, while numerous stromal nuclei remain comparatively weakly labeled. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Lung Carcinoma Tissue. Immunohistochemistry staining of FFPE human lung cancer tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in malignant epithelial cells, with brown nuclear-associated staining and variable cytoplasmic labeling. Tumor cells surrounding glandular structures show heterogeneous staining intensity, while intervening stromal cells are comparatively weakly labeled. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Human Liver Carcinoma Tissue. Immunohistochemistry staining of FFPE human liver cancer tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G expression is demonstrated by heterogeneous brown staining in tumor cells, involving both nuclear and cytoplasmic compartments. Some cells show relatively strong nuclear-associated labeling, while others exhibit weaker diffuse staining. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Mouse Brain Tissue. Immunohistochemistry staining of FFPE mouse brain tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is detected in numerous cell nuclei throughout the examined brain region, with variable staining intensity and relatively limited cytoplasmic labeling. The surrounding neuropil exhibits weak background staining. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IHC in Rat Brain Tissue. Immunohistochemistry staining of FFPE rat brain tissue using rabbit polyclonal PPIG antibody at 2 ug/ml. Cyclophilin G immunoreactivity is observed in scattered cells, with predominantly nuclear staining of varying intensity. Many surrounding cells exhibit weak or undetectable labeling, producing a heterogeneous distribution of positive nuclei. Heat-induced antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC and DAB-based detection.

PPIG Antibody by IF in Human SiHa Cells with Alpha-Tubulin Counterstaining. Immunofluorescence staining of human SiHa cells using rabbit polyclonal PPIG antibody at 5 ug/ml. Cyclophilin G immunoreactivity is visualized in green (Fluoro488), with prominent nuclear enrichment and additional diffuse staining in some cytoplasmic regions. Alpha-tubulin is detected in red (Cy3), highlighting the cytoplasmic microtubule network. Enzyme-based antigen retrieval was performed for 15 minutes, followed by overnight primary antibody incubation at 4oC and fluorescent secondary antibody detection.

PPIG Antibody by WB in Human, Rat, and Mouse Cell Lysates. Western blot analysis of Cyclophilin G in human K562 (lane 1), HEL (lane 2), HepG2 (lane 3), and RT4 (lane 4) cells; rat RH35 (lane 5) and C6 (lane 6) cells; and mouse Hepa1-6 (lane 7) and Neuro-2a (lane 8) cells. Samples containing 30 ug of protein per lane were separated by 10% SDS-PAGE under reducing conditions. The rabbit polyclonal antibody was used at 0.5 ug/ml and detected a prominent band at approximately 120 kDa across all eight samples, compared with the calculated molecular weight of 88.6 kDa. This higher apparent molecular weight is consistent with independent reports of PPIG migration near 110 kDa and may reflect anomalous electrophoretic mobility associated with its serine/arginine-rich regions and phosphorylation. Additional lower-molecular-weight bands are visible in several lanes.