- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
NF-E2 p45 Antibody / Nuclear Factor Erythroid 2 Antibody (RQ8090)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ8090 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q16621 |
| Applications | Western Blot : 0.5-1ug/ml Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This NF-E2 p45 Antibody / Nuclear Factor Erythroid 2 Antibody is available for research use only. |
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NF-E2 p45 Antibody / Nuclear Factor Erythroid 2 Antibody detects NFE2, the 45 kDa large subunit of the nuclear factor erythroid 2 transcription factor complex. NFE2 belongs to the cap'n'collar basic leucine zipper family of transcriptional regulators and forms heterodimers with small Maf proteins. These complexes bind regulatory DNA sequences and participate in transcriptional programs involved in hematopoietic differentiation, erythroid gene expression and megakaryocyte development.
NFE2 was originally identified through its role in erythroid-specific transcription. The NF-E2 complex interacts with regulatory elements associated with globin gene expression and contributes to transcriptional control during erythroid maturation. This activity makes NF-E2 p45 relevant to studies of erythropoiesis, hemoglobin production and the molecular mechanisms controlling differentiation of hematopoietic progenitor cells.
NFE2 also has important functions in the megakaryocytic lineage. Expression and activity of the transcription factor contribute to late stages of megakaryocyte maturation, proplatelet formation and platelet production. Alterations in NFE2 expression or function are therefore investigated in disorders involving abnormal erythroid or megakaryocytic development. NFE2 has received particular attention in myeloproliferative neoplasms, where increased expression can accompany abnormal proliferation and differentiation of hematopoietic cells.
As a transcriptional regulator, NFE2 functions through interactions with DNA and other regulatory proteins to influence lineage-specific gene expression. Studying NF-E2 p45 can therefore provide insight into the relationship between transcription factor activity, hematopoietic cell differentiation and disease-associated changes in gene regulation. Its expression in erythroid-lineage cells such as K562 also makes the protein useful for experimental studies of hematopoietic transcriptional control.
This rabbit polyclonal antibody was generated against recombinant human NFE2 spanning amino acids 20-373 and purified by antigen affinity chromatography. It has been evaluated by western blot using human K562 erythroleukemia and HepG2 hepatocellular carcinoma cell lysates, with NFE2 observed at approximately 41-45 kDa. NSJ Bioreagents supplies antibodies for hematopoiesis, transcription factor and cell differentiation research. An NF-E2 p45 Antibody can be used to investigate erythroid differentiation, megakaryocyte biology, hematopoiesis and NFE2-dependent transcriptional regulation.
For a recombinant rabbit monoclonal reagent with broader application validation, see our NFE2 Antibody 31N33 / Erythroid Transcription Factor Marker page.
Optimal dilution of the NF-E2 p45 Antibody / Nuclear Factor Erythroid 2 Antibody should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids E20-D373) was used as the immunogen for the NF-E2 p45 Antibody.
After reconstitution, the NF-E2 p45 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
NF-E2 p45 antibody, Nuclear Factor Erythroid 2 antibody, NFE2 antibody, Nuclear Factor Erythroid-Derived 2 antibody, NF-E2 antibody
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