UBE3C Antibody / Ubiquitin Protein Ligase E3C Antibody (FY13503)

UBE3C Antibody in Human SiHa Cervical Cancer Cells IF. Immunofluorescence staining of human SiHa cervical cancer cells with UBE3C Antibody / Ubiquitin Protein Ligase E3C Antibody at 5 ug/ml. Enzyme-mediated antigen retrieval was performed for 15 min, followed by blocking with 10% goat serum. Cells were incubated with the primary antibody overnight at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1:500 for 30 min at 37oC, and nuclei were counterstained with DAPI (blue). UBE3C staining (green) is observed predominantly in the cytoplasmic region. This UBE3C Antibody enables visualization of Ubiquitin Protein Ligase E3C expression and localization in human cervical cancer cells.

UBE3C Antibody in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with UBE3C Antibody / Ubiquitin Protein Ligase E3C Antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The tissue was incubated with the primary antibody overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody for 30 min at 37oC and DAB detection. UBE3C staining is observed in the lung tumor cells. This UBE3C Antibody demonstrates detection of Ubiquitin Protein Ligase E3C in human lung cancer tissue.

UBE3C Antibody in Human, Rat and Mouse Samples WB. Western blot testing of UBE3C Antibody / Ubiquitin Protein Ligase E3C Antibody using lysates from 1) human HepG2 cells, 2) human U251 glioma cells, 3) human SiHa cervical cancer cells, 4) human RT4 bladder cancer cells, 5) rat small intestine, 6) rat liver, 7) mouse small intestine and 8) mouse liver. The antibody was used at 0.5 ug/ml overnight at 4oC. A prominent band is detected at approximately 70 kDa. The predicted molecular weight of full-length UBE3C is approximately 124 kDa.