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Home >> Antibodies >> Melanoma Antibody / gp100 / PMEL17

Melanoma Antibody / gp100 / PMEL17 [clone NKI-beteb] (V2254)

  Catalog No Formulation Size Price (USD)  
Image V2254-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V2254-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V2254SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
IHC testing of melanoma tissue stained with Melanoma antibody (NKI-beteb). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Melamona antibody (clone NKI-beteb). These results demonstrate the foremost specificity of the NKI-beteb mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Melanoma antibody (clone NKI-beteb) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name NKI-beteb
Purity Protein G affinity chromatography
Gene ID 6490
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Melanoma gp100 antibody is available for research use only.
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Description

By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.

Application Notes

Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

Immunogen

Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).

Storage

Store the Melanoma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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