- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant PMEL17 Antibody / Melanoma gp100 [clone rPMEL17/6821] (V9583)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V9583-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V9583-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9583SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rPMEL17/6821 |
| Purity | Protein A/G affinity |
| UniProt | P40967 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This PMEL17 antibody is available for research use only. |
|
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PMEL17 antibody recognizes Premelanosome protein, a melanocyte lineage-specific type I transmembrane glycoprotein encoded by the PMEL gene and commonly referred to as Melanoma gp100, SILV, Pmel17, and Silver locus protein homolog. PMEL17 is primarily localized to the membrane of early-stage melanosomes within melanocytes and melanoma cells, where it undergoes regulated proteolytic processing to form fibrillar matrix structures. As a structural component of the melanosome, PMEL17 plays a critical role in melanin deposition and pigment organelle maturation.
Recombinant PMEL17 antibody detects a heavily glycosylated protein that is synthesized in the endoplasmic reticulum, trafficked through the Golgi apparatus, and targeted to stage I and II melanosomes. Within these organelles, PMEL17 is cleaved into functional fragments that assemble into amyloid-like fibrils, providing a scaffold for eumelanin polymerization. This fibrillar matrix is essential for proper pigmentation and protects melanocytes from the cytotoxic effects of reactive melanin intermediates.
Functionally, PMEL17 is a defining marker of melanocytic differentiation. Expression is observed in normal melanocytes of the skin, uveal tract, and hair follicles, as well as in the majority of primary and metastatic melanomas. Because of its lineage-restricted expression pattern, PMEL17 is widely used in diagnostic pathology to help identify melanocytic lesions and to distinguish melanoma from non-melanocytic tumors. The protein is not broadly expressed in most non-melanocytic tissues, supporting its utility as a selective marker in research applications.
The PMEL gene is located on chromosome 12 and belongs to a small group of melanosome-associated proteins that coordinate pigment synthesis and organelle biogenesis. Disruption of PMEL processing or fibril formation can impair melanosome maturation and alter pigmentation phenotypes. In melanoma biology, PMEL17 expression reflects melanocytic lineage commitment and may vary with tumor differentiation status.
The Recombinant PMEL17 antibody (clone rPMEL17/6821) is suitable for detecting PMEL protein expression in research applications. This PMEL17 antibody supports studies of melanocyte biology, melanosome structure, pigmentation pathways, and melanoma characterization.
Optimal dilution of the PMEL17 antibody should be determined by the researcher.
Recombinant full-length human protein was used as the immunogen for the PMEL17 antibody.
Aliquot the PMEL17 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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