Melanocyte Lineage Antibody / PMEL [clone PMEL/7554R] (V5997)

Analysis of HuProt Protein Array (21,000+ full-length human proteins) with Melanocyte Lineage/PMEL protein antibody (clone PMEL17/7554R). Z-score: Signal strength (in SD above the mean) of antibody binding to each protein. S-score: Difference in Z-scores between the top target and the next best hit, indicating relative specificity. An antibody is considered specific if S ≥ 2.5. Example: Binding to protein X (Z = 43) vs protein Y (Z = 14) → S = 29.

Immunohistochemistry analysis of Melanocyte Lineage / PMEL antibody in human melanoma tissue. FFPE human melanoma sections show focal HRP-DAB brown cytoplasmic staining in scattered tumor cells, consistent with premelanosome-associated localization of PMEL in melanocytic cells. Adjacent tumor cells demonstrate weak or minimal staining, reflecting heterogeneous expression within the lesion. The inset negative control, in which PBS was used in place of primary antibody, shows no specific brown chromogenic signal. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by boiling for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.