Melanoma gp100 Antibody Microarray Specificity Validation / PMEL Antibody [clone PMEL/2039] (V3929)

Melanoma gp100 Antibody with Microarray Specificity Validation (clone PMEL/2039). Immunohistochemistry analysis of human skin tissue stained with Melanoma gp100 Antibody Microarray Specificity Validation clone PMEL/2039. FFPE skin sections show focal HRP-DAB brown cytoplasmic staining in scattered melanocytes located along the basal layer of the epidermis, consistent with expression of Premelanosome protein PMEL, also known as gp100, a melanosome-associated glycoprotein characteristic of melanocytic lineage. Keratinocytes within the epidermis and underlying dermal structures show minimal background staining. Heat-induced epitope retrieval was performed in 10mM citrate buffer pH 6 by boiling for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

IHC testing of human melanoma with Melanoma gp100 antibody (clone PMEL/2039). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Melanoma gp100 Antibody Microarray Specificity Validation (clone PMEL/2039). HuProt protein microarray specificity analysis using Melanoma gp100 Antibody Microarray Specificity Validation clone PMEL/2039 against a protein array containing more than 19,000 full-length human proteins. The antibody shows strongest binding to PMEL (Premelanosome protein, also known as gp100), demonstrating highly selective recognition of the intended target compared with other proteins on the array. Z score represents the signal strength produced when the antibody binds to each protein on the array and is expressed as standard deviations above the mean signal of all proteins tested. When proteins are ranked by Z score, the S score represents the difference between adjacent Z scores and therefore reflects the relative specificity of the antibody for its intended target. These microarray results demonstrate the strong target specificity of clone PMEL/2039 for PMEL in large-scale protein microarray screening.

SDS-PAGE analysis of purified, BSA-free Melanoma gp100 antibody (clone PMEL/2039) as confirmation of integrity and purity.

Melanoma gp100 Antibody (clone PMEL/2039). Western blot analysis of human COLO-38 melanoma cell lysate using Melanoma gp100 Antibody Microarray Specificity Validation clone PMEL/2039. Lane 1: human COLO-38 cell lysate. A prominent band is detected at approximately 100 kDa, consistent with the glycosylated precursor form of Premelanosome protein PMEL, also known as gp100. PMEL is synthesized as an approximately 100 kDa glycosylated precursor that undergoes proteolytic cleavage to generate an approximately 60-64 kDa M-alpha fragment and an approximately 26 kDa M-beta fragment. The M-alpha fragment is further processed into approximately 34-38 kDa and approximately 26 kDa fragments that assemble into the fibrillar matrix of developing melanosomes in melanocytic cells.