PMEL17 Antibody Glycosylated Protein / Melanoma gp100 Antibody [clone PMEL/2037] (V3930)

PMEL17 Antibody Glycosylated Protein. Immunohistochemistry analysis of human melanoma tissue stained with PMEL17 Antibody Glycosylated Protein clone PMEL/2037. Tumor cells display strong cytoplasmic HRP-DAB brown chromogenic staining consistent with expression of Premelanosome protein PMEL, also known as gp100, a melanosome-associated glycoprotein characteristic of melanocytic lineage. Positive staining highlights melanoma tumor cells distributed throughout the tissue section, while surrounding stromal elements show minimal background staining. Heat-induced epitope retrieval was performed by boiling sections in 10mM citrate buffer pH 6 for 10-20 minutes followed by cooling at room temperature prior to staining.

PMEL17 Antibody Glycosylated Protein. Immunohistochemistry analysis of human skin tissue stained with PMEL17 Antibody Glycosylated Protein clone PMEL/2037. Brown HRP-DAB chromogenic staining is observed in melanocytic cells located along the basal layer of the epidermis, consistent with expression of Premelanosome protein PMEL, also known as gp100, a melanosome-associated glycoprotein characteristic of melanocytic lineage. The staining highlights scattered melanocytes within the epidermal basal layer while surrounding keratinocytes and dermal cells remain largely negative. Heat-induced epitope retrieval was performed by boiling sections in 10mM citrate buffer pH 6 for 10-20 minutes followed by cooling at room temperature prior to staining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PMEL17 antibody (clone PMEL/2037). These results demonstrate the foremost specificity of the PMEL/2037 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PMEL17 antibody (clone PMEL/2037) as confirmation of integrity and purity.

PMEL17 Antibody Glycosylated Protein. Western blot analysis of human COLO-38 melanoma cell lysate using PMEL17 Antibody Glycosylated Protein clone PMEL/2037. Lane 1: human COLO-38 cell lysate. A band is detected at approximately 100 kDa, consistent with the glycosylated precursor form of Premelanosome protein PMEL, also known as gp100. PMEL is synthesized as a glycosylated precursor that migrates near approximately 100 kDa before undergoing proteolytic cleavage into an approximately 60-64 kDa M-alpha fragment and an approximately 26 kDa M-beta fragment. The M-alpha fragment is further processed into approximately 34-38 kDa and approximately 26 kDa fragments that assemble into the fibrillar matrix of developing melanosomes in melanocytic cells.