Melanoma gp100 Antibody Protein Microarray Validated / PMEL Antibody [clone PMEL/2038] (V3931)

Melanoma gp100 Antibody Protein Microarray Validated (clone PMEL/2038). Immunohistochemistry analysis of human melanoma tissue stained with Melanoma gp100 Antibody Protein Microarray Validated clone PMEL/2038. FFPE melanoma sections show strong HRP-DAB brown cytoplasmic and membranous staining in tumor cells consistent with expression of Premelanosome protein PMEL, also known as gp100, a melanosome-associated glycoprotein characteristic of melanocytic lineage. Staining highlights cohesive nests and sheets of melanoma tumor cells, while adjacent stromal regions show minimal background staining. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA pH 9 by boiling for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

IHC testing of human skin with Melanoma gp100 antibody (clone PMEL/2038). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Melanoma gp100 Antibody Protein Microarray Validated (clone PMEL/2038). HuProt protein microarray specificity analysis using Melanoma gp100 Antibody Protein Microarray Validated clone PMEL/2038 against a microarray containing more than 19,000 full-length human proteins. The antibody shows strongest binding to PMEL17 (Premelanosome protein, also known as gp100), demonstrating selective target recognition compared with other proteins on the array. Z score represents the signal strength produced when the antibody binds to each protein on the array and is expressed as standard deviations above the mean signal generated across the array. When proteins are ranked by Z score, the S score represents the difference between adjacent Z scores and therefore reflects the relative specificity of the antibody for its intended target. These results demonstrate the strong specificity of clone PMEL/2038 for PMEL17 in large-scale protein microarray screening.

SDS-PAGE analysis of purified, BSA-free Melanoma gp100 antibody (clone PMEL/2038) as confirmation of integrity and purity.

Western blot testing of human COLO-38 cell lysate with microarray validated Melanoma gp100 antibody (clone PMEL/2038). The ~100 kDa glycosylated PMEL precursor is proteolytically cleaved into an ~60-64 kDa M-alpha fragment and an ~26 kDa M-beta fragment. The M-alpha fragment is subsequently processed into ~34-38 kDa and ~26 kDa fragments that assemble into the fibrillar matrix of melanosomes.