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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for IL-10

IL10 Antibody / Interleukin 10 (clone AOIG-9)

Catalog No : FY12858
Applications : WB, ICC, IF, FACS
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of IL10 using anti-IL10 antibody. Lane 1: human U251 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot of IL-10 in human and rodent spleen lysates shows a band at ~17-18 kDa, consistent with the mature, processed IL-10 monomer after removal of the signal peptide. The apparent size is slightly lower than the 21 kDa precursor predicted from the unprocessed sequence.
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IL-10 Antibody (clone IL10/2651R)

Catalog No : V7295
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human prostate carcinoma with recombinant IL-10 antibody (clone IL10/2651R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IL26 Antibody / Interleukin 26 (clone 31I11)

Catalog No : FY12058
Applications : WB
Reactivity : Human
Format : Liquid
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Western blot analysis of IL26 in His-tagged IL26 transfected cell lysate using IL26 antibody.
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IL22 Antibody / Interleukin 22 (clone 31I09)

Catalog No : FY12351
Applications : WB
Reactivity : Human
Format : Liquid
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Western blot analysis of IL22 in recombinant human IL22 protein cell lysate using IL22 antibody.
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IL-10 Antibody

Catalog No : R30116
Applications : WB, IHC-P, ELISA (protein)
Reactivity : Human
Format : Antigen affinity purified
IHC staining of FFPE human spleen tissue with human IL-10 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IL-10 Antibody

Catalog No : F51527
Applications : WB, IHC, FACS, ELISA
Reactivity : Human
Pred. Reactivity :Primate
Format : Antigen affinity purified
IL-10 antibody flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IL-10 Antibody (clone IL10/8711)

Catalog No : V4788
Applications : IHC-P
Reactivity : Human
Format : Purified
IHC staining of FFPE human prostate cancer tissue with Interleukin 10 antibody (clone IL10/8711). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Il-10 Antibody

Catalog No : R30118
Applications : WB, IHC-P, ELISA (protein)
Reactivity : Rat
Format : Antigen affinity purified
IHC staining of FFPE rat spleen tissue with Il10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Il-10 Antibody

Catalog No : RQ6187
Applications : IHC-P, Direct ELISA
Reactivity : Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE rat spleen with Interleukin 10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Il-10 Antibody

Catalog No : R30117
Applications : WB, ELISA (protein)
Reactivity : Mouse
Format : Antigen affinity purified
Western blot testing of mouse Il-10 antibody and Lane 1: Recombinant mouse protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng
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