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Home >> Antibodies >> IL-10 Antibody IL10/2651R / CSIF Antibody

IL-10 Antibody IL10/2651R / CSIF Antibody [clone IL10/2651R] (V7295)

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  Catalog No Formulation Size Price (USD)  
Image V7295-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7295-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7295SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V7295IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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IL-10 Antibody IL10/2651R Staining in Human Prostate Carcinoma IHC. Immunohistochemistry staining of FFPE human prostate carcinoma tissue using IL-10 Antibody (clone IL10/2651R) shows prominent brown staining in malignant glandular epithelial cells, with predominantly cytoplasmic and membranous signal. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. The distinct epithelial staining demonstrates detection of IL-10 using this CSIF Antibody in human prostate carcinoma tissue.
IL-10 Antibody IL10/2651R Staining in Human Endocervical Carcinoma IHC. Immunohistochemistry staining of FFPE human endocervical carcinoma tissue using IL-10 Antibody (clone IL10/2651R) shows strong brown staining within malignant glandular epithelial structures, while much of the surrounding stromal tissue remains comparatively lightly stained. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. This CSIF Antibody demonstrates prominent IL-10 detection in human endocervical carcinoma tissue.
IL-10 Antibody IL10/2651R Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of 2.5 ug purified, BSA-free IL-10 Antibody (clone IL10/2651R) shows the intact antibody migrating above 98 kDa under non-reducing conditions. Under reducing conditions, distinct immunoglobulin heavy and light chains are visible at approximately 50 kDa and 28 kDa, respectively. The expected migration pattern supports the integrity and purity of this recombinant CSIF Antibody preparation.
IL-10 Antibody IL10/2651R Detects IL-10 in Human Spleen and Cell Lines WB. Western blot analysis of human spleen, Raji B lymphocyte, and K-562 chronic myelogenous leukemia cell lysates using IL-10 Antibody (clone IL10/2651R) shows discrete major bands at approximately 29 kDa in all three samples, with the strongest signal in K-562 cells. Although monomeric IL-10 is approximately 18-20 kDa, IL-10 forms homodimers and higher apparent molecular-weight species in the approximately 30-35 kDa range have been reported. The consistent migration across the three lysates supports detection of IL-10 using this CSIF Antibody, with little visible nonspecific background.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name IL10/2651R
Purity Protein A affinity chromatography
UniProt P22301
Localization Cell surface, cytoplasmic, secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Blot : 2-4ug/ml
Limitations This IL-10 Antibody IL10/2651R / CSIF Antibody is available for research use only.
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Description

IL-10 Antibody IL10/2651R recognizes interleukin 10 (IL-10), an immunoregulatory cytokine encoded by the IL10 gene. IL-10 was originally characterized as cytokine synthesis inhibitory factor (CSIF) because of its ability to suppress cytokine production by activated immune cells. It is now recognized as an important regulator of immune homeostasis that limits excessive inflammatory responses while helping protect tissues from immune-mediated damage.

IL-10 signals through a heterotetrameric receptor complex composed of IL10RA and IL10RB subunits. Ligand binding activates the receptor-associated kinases JAK1 and TYK2, leading predominantly to phosphorylation and activation of STAT3. The resulting transcriptional program regulates genes involved in inflammatory signaling, immune-cell activation, and feedback control of cytokine responses. Through these mechanisms, IL-10 can suppress production of pro-inflammatory mediators and modulate antigen-presenting cell activity.

IL-10 is produced by multiple immune-cell populations, including regulatory and conventional T cells, B cells, macrophages, dendritic cells, and other leukocytes. Its effects are particularly important in controlling the magnitude and duration of immune responses. Dysregulated IL-10 production or signaling is studied in chronic inflammation, autoimmune disease, infectious disease, cancer, and disorders involving impaired immune regulation. Depending on the biological setting, IL-10 can influence both innate and adaptive immune responses, making it an important subject in research examining mechanisms that balance inflammation with immune tolerance.

Clone IL10/2651R is a recombinant rabbit monoclonal antibody generated against full-length recombinant human IL-10 protein. As a CSIF Antibody, clone IL10/2651R provides a defined reagent for investigating the cytokine historically identified as cytokine synthesis inhibitory factor and its role in controlling inflammatory and immune responses. NSJ Bioreagents supplies IL10/2651R for research use only.

An IL-10 Antibody can be used to investigate IL10 expression and molecular mechanisms connecting cytokine signaling, inflammatory regulation, immune homeostasis, and adaptive and innate immune responses.

Explore our Immunology Antibodies page for additional antibodies targeting cytokines and regulators of immune activation, inflammatory responses, and immune homeostasis.

Application Notes

Optimal dilution of the IL-10 Antibody IL10/2651R / CSIF Antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length human protein was used as the immunogen for the IL-10 Antibody IL10/2651R.

Storage

Store the IL-10 Antibody IL10/2651R at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

IL-10 antibody, IL10 antibody, interleukin 10 antibody, interleukin-10 antibody, CSIF antibody, cytokine synthesis inhibitory factor antibody, cytokine synthesis inhibitory factor 10 antibody, TGIF antibody

Related Products

   1.   View all IL-10 antibodies

   2.   View all recombinant antibodies

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