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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 1521 to 1530
    NCAM / CD56 Antibody (clone NCAM1/8392R)
    Catalog No : V4193
    Applications : IHC-P, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD56 Antibody Recombinant Rabbit MAb NCAM1/8392R. Immunohistochemistry of FFPE human colon tissue demonstrates membranous and cytoplasmic staining in scattered neural and neuroendocrine-associated cell clusters consistent with expression of Neural cell adhesion molecule 1 (NCAM1), also known as CD56. Brown chromogenic signal highlights CD56-positive cells within localized structures embedded in the surrounding stromal tissue, while most epithelial and stromal cells show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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    NCAM / CD56 Antibody (clone NCAM1/9074R)
    Catalog No : V4194
    Applications : IHC-P, WB
    Reactivity : Human, Mouse
    Format : Purified
    Recrabbitmono
    NCAM Antibody for WB (clone NCAM1/9074R). Western blot analysis of human brain, mouse brain, and rat brain tissue lysates demonstrates multiple bands between approximately 120-180 kDa, consistent with known isoforms of Neural cell adhesion molecule 1 (NCAM1), also known as CD56. These bands correspond to the major NCAM protein variants produced by alternative splicing and post-translational modification. Detection of NCAM1 across human, mouse, and rat brain samples reflects the strong expression of this adhesion molecule in neural tissues and supports its established role in neuronal development and cell-cell adhesion.
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    CD3 epsilon Antibody (clone C3e/8115R)
    Catalog No : V4205
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD3 epsilon Antibody Recombinant Rabbit Monoclonal. Immunohistochemistry analysis of CD3 Epsilon / CD3E antibody in FFPE human tonsil tissue using CD3 epsilon Antibody Recombinant Rabbit Monoclonal Clone C3e/8115R. Strong HRP-DAB brown membranous and cytoplasmic staining is observed in T lymphocytes within interfollicular and paracortical regions, consistent with T-cell zone distribution, while surrounding non-lymphoid cells show minimal background. The staining highlights dense T-cell populations within lymphoid architecture and demonstrates clear signal definition with low non-specific staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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    CD3 epsilon Antibody (clone C3e/8116R)
    Catalog No : V4212
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD3e Antibody. Immunohistochemistry analysis of CD3 Epsilon / CD3E antibody in FFPE human tonsil tissue using CD3e Antibody / T Cell Activation Marker Antibody with Clone C3e/8116R. Strong HRP-DAB brown membranous and cytoplasmic staining is observed in T lymphocytes within interfollicular and paracortical regions, consistent with activated T-cell distribution, while surrounding non-lymphoid cells show minimal background. The staining highlights CD3 epsilon as a key mediator of T cell activation within lymphoid tissue architecture. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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    CXCR5 Antibody / BLR1 / MDR15 (clone CXCR5/8146R)
    Catalog No : V4213
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of CXCR5 antibody in human tonsil tissue. FFPE human tonsil sections show strong membranous HRP-DAB brown staining in lymphoid cells within germinal centers, consistent with CXCR5, also known as CD185, expression in follicular B cells and T follicular helper cells. Interfollicular regions show comparatively reduced staining. The inset negative control, in which PBS was used in place of primary antibody, shows no specific brown chromogenic signal. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.
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    CXCR5 Antibody / BLR1 / MDR15 (clone CXCR5/8279R)
    Catalog No : V4214
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of CD185 antibody in human tonsil tissue. FFPE human tonsil sections show strong membranous HRP-DAB brown staining in lymphoid cells within germinal centers, consistent with CXCR5, also known as CD185, expression in follicular B cells and T follicular helper cells. Interfollicular regions demonstrate comparatively lower staining intensity. The inset negative control, in which PBS was used in place of primary antibody, shows no specific brown chromogenic signal. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.
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    CD27 Antibody (clone LPFS2/8574R)
    Catalog No : V4221
    Applications : IHC-P, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression in human B-cell lymphoma cell lines using CD27 Antibody / Tumor Immune Microenvironment Marker Antibody. Lane 1: Raji cell lysate, Lane 2: Ramos cell lysate. A band is detected at approximately 50–60 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight of approximately 29 kDa for the core protein. Additional lower molecular weight signal may represent unmodified or partially processed forms of CD27. Detection of CD27 in Raji and Ramos cells aligns with expression in B-cell lineage populations relevant to tumor-associated immune environments.
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    CD27 Antibody (clone LPFS2/8838R)
    Catalog No : V4224
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD27 Antibody for Human LN IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human lymph node tissue using CD27 Antibody / Immune Checkpoint Pathway Antibody clone LPFS2/8838R. Prominent membranous HRP-DAB brown staining is observed in lymphocyte populations within follicular and paracortical regions, consistent with CD27 expression on activated and regulatory T and B cell subsets involved in immune signaling, while surrounding stromal elements remain largely negative. The staining pattern highlights immune regulatory compartments and supports evaluation of checkpoint-related signaling within lymphoid tissue architecture. Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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    NSE Antibody / Neuron Specific Enolase (clone ENO/8253R)
    Catalog No : V4230
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human pancreas tissue with NSE antibody (clone ENO/8253R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    NSE Antibody / Neuron Specific Enolase (clone ENO/8614R)
    Catalog No : V4231
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human pancreas tissue with NSE antibody (clone ENO/8614R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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