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Home >> Antibodies >> Recombinant Retinoblastoma Antibody RB1/7080R / RB1 Rabbit MAb

Recombinant Retinoblastoma Antibody RB1/7080R / RB1 Rabbit MAb [clone RB1/7080R] (V4327)

  Catalog No Formulation Size Price (USD)  
Image V4327-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4327-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4327SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Recombinant Retinoblastoma Antibody RB1/7080R Immunohistochemistry. IHC staining of FFPE human bladder carcinoma with recombinant Retinoblastoma antibody (clone RB1/7080R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Retinoblastoma antibody (clone RB1/7080R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name RB1/7080R
Purity Protein A/G affinity
UniProt P06400
Localization Nucleus
Applications Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Limitations This Recombinant Retinoblastoma Antibody RB1/7080R is available for research use only.
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Description

Recombinant Retinoblastoma Antibody RB1/7080R recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Explore our Retinoblastoma Antibody FCD-18, a rabbit monoclonal antibody targeting RB1, for an additional option with Western blot, immunohistochemistry, and immunofluorescence validation.

Application Notes

Optimal dilution of the Recombinant Retinoblastoma Antibody RB1/7080R should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant Retinoblastoma antibody.

Storage

Aliquot the recombinant Retinoblastoma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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