Recombinant IgD Heavy Chain Antibody IGHD/8228R / Immunoglobulin D Heavy Chain Antibody [clone IGHD/8228R] (V4340)

Recombinant IgD Heavy Chain Antibody IGHD/8228R immunohistochemistry analysis of human tonsil tissue. Formalin-fixed paraffin-embedded human tonsil tissue stained with Recombinant IgD Heavy Chain Antibody IGHD/8228R shows HRP-DAB brown chromogenic staining in B lymphocytes within lymphoid follicles. Positive cells are distributed primarily in mantle zone regions surrounding germinal centers and display membranous and cytoplasmic staining consistent with IgD expression in mature naive B cells. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Recombinant IgD Heavy Chain Antibody IGHD/8228R immunohistochemistry analysis of human tonsil tissue. Formalin-fixed paraffin-embedded human tonsil tissue stained with Recombinant IgD Heavy Chain Antibody IGHD/8228R shows HRP-DAB brown chromogenic staining in B lymphocytes within lymphoid follicles, with positive cells distributed predominantly in mantle zone regions surrounding germinal centers. Staining is localized to the cell membrane and cytoplasm of IgD-positive B cells consistent with expression of Immunoglobulin D in mature naive B lymphocytes. The inset shows the negative control in which PBS was used in place of the primary antibody, demonstrating absence of specific staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.