- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Creatine kinase B Antibody / Brain Creatine Kinase / CKBB [clone CKBB/8608R] (V4377)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4377-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
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V4377-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V4377SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CKBB/8608R |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This Creatine kinase B antibody is available for research use only. |
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Creatine kinase B antibody detects Creatine kinase B-type, also known as brain creatine kinase or CKBB, an enzyme that catalyzes the reversible transfer of phosphate between creatine and ATP to generate phosphocreatine and ADP. The UniProt recommended name is Creatine kinase B-type (CKB). This enzyme provides a rapid energy buffering system in tissues with fluctuating ATP demands, particularly the brain, retina, and smooth muscle.
In neurons, Creatine kinase B antibody identifies a cytosolic enzyme that supports continuous ATP supply during synaptic transmission and ion transport. CKB is a critical component of the phosphocreatine shuttle, coupling energy production in mitochondria to ATP utilization sites such as Na+/K+-ATPase pumps, synaptic vesicles, and the cytoskeleton. By ensuring rapid phosphate transfer, CKBB maintains neuronal excitability, neurotransmitter release, and membrane potential stability. This process allows brain cells to operate efficiently even during intense electrical activity or short-term energy deprivation.
The CKB gene resides on chromosome 14q32.33 and encodes a 381-amino acid enzyme functioning as a homodimer. Each subunit contains active sites for ATP and creatine binding and relies on magnesium ions for catalysis. CKB expression is highest in brain and neural-derived tissues but is also detected in kidney, testis, and placenta. When brain tissue is injured or oxygen-deprived, CKBB leaks into the bloodstream and cerebrospinal fluid, making it a biochemical marker for neural damage and hypoxia. Dysregulated CKB expression has been observed in certain cancers, where it supports tumor energy metabolism and resistance to oxidative stress.
Creatine kinase B antibody is widely used in research exploring cellular energy homeostasis, oxidative stress response, and neurodegeneration. The enzymeâs distribution near ion channels and vesicular transport systems links it directly to energy turnover at key functional sites. In knockout and silencing studies, loss of Ckb leads to impaired energy buffering, decreased neuronal performance, and increased vulnerability to ischemic conditions. These findings underscore its indispensable role in neural resilience and synaptic function.
Outside the nervous system, CKB contributes to energy regulation in sperm motility, sensory perception, and muscle contractility. The enzyme is modulated by post-translational mechanisms such as phosphorylation and oxidation and transcriptionally controlled by CREB and Sp1. Through its central role in energy transfer, CKB acts as a stabilizing element across multiple physiological systems. NSJ Bioreagents provides Creatine kinase B antibody reagents validated for use in metabolic, neurological, and cancer-related research applications.
Optimal dilution of the Creatine kinase B antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the Creatine kinase B antibody.
Aliquot the Creatine kinase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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