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  • separatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
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Signal Transduction Antibodies

Home >> Antibodies >> Signal Transduction Antibodies

Signal Transduction Antibodies

NSJ Bioreagents offers the following antibodies in the area of Signal Transduction:
Found all antibodies, displaying 10921 to 10925
CLIC4 Antibody / Chloride intracellular channel protein 4 (clone 30C24)
Catalog No : FY12005
Applications : WB, IHC, ICC, IF, IP, FACS
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Western blot analysis of CLIC4 using anti-CLIC4 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for CLIC4 at approximately 29 kDa. The expected band size for CLIC4 is at 29 kDa.
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LAT Antibody / Linker for activation of T-cells family member 1
Catalog No : FY12034
Applications : ELISA, WB, FACS
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of HEL cells using anti-LAT antibody. Overlay histogram showing HEL cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LAT antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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PABPN1 Antibody / Polyadenylate-binding protein 2
Catalog No : FY12099
Applications : WB, IHC, IF, ICC/IF, IP, FACS
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of PABPN1 using anti-PABPN1 antibody (red). PABPN1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-PABPN1 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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KEAP1 Antibody / Kelch-like ECH-associated protein 1
Catalog No : FY12106
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
IF analysis of KEAP1 using anti-KEAP1 antibody (green). KEAP1 was detected in an immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-KEAP1 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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MAP4K3 Antibody / MEKKK3 / Mitogen-activated protein kinase kinase kinase kinase 3
Catalog No : FY12268
Applications : WB, IHC, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of MAP4K3 using anti-MAP4K3 antibody. MAP4K3 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MAP4K3 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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