NTRK3 Antibody / Neurotrophic receptor tyrosine kinase 3 / TrkC [clone NTRK3/8796] (V5961)

IHC staining of NTRK3/Neurotrophic receptor tyrosine kinase 3 antibody (clone NTRK3/8796). FFPE human brain tissue shows HRP-DAB brown staining in neuronal cell bodies and neuropil with membranous and cytoplasmic distribution, consistent with known expression of Neurotrophic receptor tyrosine kinase 3 in central nervous system tissue. Inset: PBS instead of primary antibody; secondary only negative control. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95oC followed by cooling at RT for 20 minutes.

Western Blot Analysis of NTRK3 / TrkC antibody (clone NTRK3/8796). Human brain and rat brain tissue lysates show a dominant immunoreactive band at approximately 50 kDa, with additional higher molecular weight species visible in rat brain. The predicted molecular weight of full-length TrkC is approximately 90-95 kDa; however, NTRK3 undergoes alternative splicing and proteolytic processing, generating truncated isoforms lacking portions of the intracellular kinase domain that migrate at lower apparent molecular weights. The ~50 kDa band is consistent with a truncated TrkC isoform, while the higher bands likely represent full-length and differentially glycosylated receptor species, which commonly migrate above their predicted molecular weight due to N-linked glycosylation of the extracellular domain.

Western Blot Analysis of NTRK3 / TrkC antibody (clone NTRK3/8796). U-87 human glioblastoma cell lysate shows two major immunoreactive bands at approximately 36 kDa and 130-140 kDa. The predicted molecular weight of full-length TrkC is approximately 90-95 kDa; however, extensive glycosylation of the extracellular region can shift the apparent molecular weight to 120-140 kDa on SDS-PAGE. The lower ~36 kDa band is consistent with a truncated or processed NTRK3 isoform lacking portions of the receptor, which has been reported for kinase-deficient splice variants. The presence of both higher glycosylated and lower truncated forms reflects known isoform diversity and post-translational modification of Neurotrophic receptor tyrosine kinase 3.

SDS-PAGE Analysis of Purified NTRK3/Neurotrophic receptor tyrosine kinase 3 antibody (NTRK3/8796). Confirmation of Purity and Integrity of Antibody.