p53 Antibody DO-7 | N terminus | V2284 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2284-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2284-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2284SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2284IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC staining of FFPE normal human colon with p53 antibody (clone DO-7).

Western blot testing of 293 cell lysate with p53 antibody (clone DO-7).

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	DO-7
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	7157
Localization	Nuclear
Applications	Western blot : 0.5-1.0ug/ml Immunohistochemistry (FFPE) : 0.5-1.0ug/ml for 30 min at RT (1) Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations	This p53 antibody is available for research use only.
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Description

This antibody is specific for a 53kDa protein, which is identified as p53 suppressor gene product. The large number of prolines in its amino acid sequence causes p53 to migrate slowly in SDS-PAGE, resulting in the amino acid content-estimated 43 kDa protein appearing larger than expected. DO-7 reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intra-tubular germ cell neoplasia.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human wild type p53 expressed in E. coli was used as the immunogen for this antibody. Its epitope maps within the N-terminus portion (aa 20-25) of the p53 oncoprotein.