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Home >> Antibodies >> Anti-p53 Antibody

Anti-p53 Antibody (F48067)

  Catalog No Formulation Size Price (USD)  
Image F48067-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F48067-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
IHC analysis of FFPE human esophagus section using anti-p53 antibody; Ab was diluted at 1:100.
Anti-p53 antibody western blot analysis in 293 lysate
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P04637
Applications IHC (Paraffin) : 1:100
Western blot : 1:1000
Limitations This anti-p53 antibody is available for research use only.
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Description

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

Application Notes

Titration of the anti-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 364-393 from the human protein was used as the immunogen for this anti-p53 antibody.

Storage

Aliquot the anti-p53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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