p53 Antibody for FACS / TP53 Flow Cytometry Antibody [clone TP53/1799R] (V3513)

Description

Tumor protein p53 (TP53) is a sequence-specific transcription factor that functions as a central tumor suppressor regulating DNA damage responses, apoptosis, and genomic stability. The p53 Antibody for FACS clone TP53/1799R is a recombinant rabbit monoclonal antibody developed specifically for flow cytometry applications, enabling sensitive detection of intracellular p53 protein in individual cells during fluorescence-activated cell sorting analysis.

TP53 antibody reagents are widely used in cancer research because p53 is one of the most frequently altered proteins in human tumors. The TP53 gene, located on chromosome 17p13.1, encodes Tumor protein p53, also known as Cellular tumor antigen p53. The p53 protein contains several functional regions including an N-terminal transcriptional activation domain, a central DNA-binding domain responsible for sequence-specific transcriptional regulation, a tetramerization domain required for formation of active transcription complexes, and a regulatory C-terminal region that modulates DNA binding and protein stability.

The defining characteristic of the p53 Antibody for FACS clone TP53/1799R is its application in fluorescence-activated cell sorting workflows. Flow cytometry enables quantitative measurement of intracellular protein levels in thousands of individual cells, allowing investigators to detect shifts in p53 expression within heterogeneous cell populations. Using the p53 Antibody for FACS, researchers can identify TP53-positive cell populations and analyze changes in p53 abundance following DNA damage, oncogenic signaling, or other cellular stress responses.

Stabilization of p53 protein frequently occurs after genotoxic stress or activation of tumor suppressor pathways. In these situations, intracellular p53 levels increase and can be measured by flow cytometry analysis using antibodies designed for fluorescence-based detection. The p53 Antibody for FACS enables evaluation of these cellular responses by measuring fluorescence intensity associated with intracellular p53 staining in individual cells.

The recombinant monoclonal clone TP53/1799R therefore provides a defined reagent for detection of Tumor protein p53 during fluorescence-activated cell sorting experiments. Through use of the p53 Antibody for FACS clone TP53/1799R, researchers can investigate TP53 pathway activation, quantify p53 expression across cell populations, and monitor intracellular tumor suppressor signaling at single-cell resolution.

Application Notes

The optimal dilution of the p53 Antibody for FACS / TP53 Flow Cytometry Antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human p53 protein was used as the immunogen for this p53 Antibody for FACS / TP53 Flow Cytometry Antibody.

Storage

Store the recombinant p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

TP53 antibody, Tumor protein p53 antibody, Cellular tumor antigen p53 antibody, p53 tumor suppressor antibody