p53 Antibody for IP / Tumor Protein p53 Immunoprecipitation Antibody (R31441)

p53 Antibody for IP Human 293T Cell Immunoprecipitation. Immunoprecipitation of p53/TP53 was performed from human 293T whole cell lysate using p53 Antibody for IP, also called Tumor Protein p53 Immunoprecipitation Antibody. Lane 1 contains 30 ug of input lysate; lane 2 contains material immunoprecipitated with rabbit control IgG; lane 3 contains material immunoprecipitated with 2 ug of anti-TP53 antibody from 500 ug of lysate. Western blot detection was performed with anti-TP53 antibody at 0.5 ug/ml and a light chain-specific HRP secondary antibody. A strong band at approximately 53 kDa is present in the input and TP53 immunoprecipitate but absent from the control IgG lane, demonstrating successful enrichment of p53 from human 293T cells.

p53 Antibody for IP Human PC-9 TP53 Knockout Validation WB. Western blot analysis of human PC-9 wild-type and TP53 knockout cell lysates was performed with p53 Antibody for IP, also called Tumor Protein p53 Immunoprecipitation Antibody. Lane 1: PC-9 wild-type cell lysate; Lane 2: PC-9 TP53 knockout cell lysate. Samples were loaded at 30 ug per lane, and the antibody was used at 0.5 ug/ml overnight at 4oC. A strong p53 band is detected at approximately 53 kDa in wild-type cells and is absent from the TP53 knockout cells, supporting specific recognition of endogenous p53.

p53 Antibody for IP Human 293T and T47D Cell WB. Western blot analysis of human 293T and T47D whole cell lysates was performed with p53 Antibody for IP, also called Tumor Protein p53 Immunoprecipitation Antibody. Lane 1: 293T cell lysate; Lane 2: T47D cell lysate. Samples were loaded at 30 ug per lane, and the antibody was used at 0.5 ug/ml overnight at 4oC. A strong p53 band is detected at approximately 53 kDa in both cell lysates. This p53 Antibody demonstrates detection of endogenous TP53 in human cells and complements its validated use for immunoprecipitation.

p53 Antibody for IP Human Colon Cancer IHC. Immunohistochemistry staining of FFPE human colon cancer tissue was performed with p53 Antibody for IP, also called Tumor Protein p53 Immunoprecipitation Antibody. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0. The antibody was used at 2 ug/ml overnight at 4oC, followed by an HRP-conjugated secondary antibody and DAB detection. Positive nuclear staining is observed in populations of colonic epithelial cells. This p53 Antibody demonstrates detection of endogenous TP53 in human colon cancer tissue and complements its validated use for immunoprecipitation.

p53 Antibody for IP Human Colon Cancer Glandular Epithelium IHC. Immunohistochemistry staining of FFPE human colon cancer tissue was performed with p53 Antibody for IP, also called Tumor Protein p53 Immunoprecipitation Antibody. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0. The antibody was used at 2 ug/ml overnight at 4oC, followed by an HRP-conjugated secondary antibody and DAB detection. Positive nuclear staining is observed in populations of malignant glandular epithelial cells. This p53 Antibody demonstrates detection of endogenous TP53 in human colon cancer and complements its validated use for immunoprecipitation.

p53 Antibody for IP Human Ovarian Cancer IHC. Immunohistochemistry staining of FFPE human ovarian cancer tissue was performed with p53 Antibody for IP, also called Tumor Protein p53 Immunoprecipitation Antibody. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0. The antibody was used at 2 ug/ml overnight at 4oC, followed by an HRP-conjugated secondary antibody and DAB detection. Positive nuclear staining is observed in populations of tumor epithelial cells. This p53 Antibody demonstrates detection of endogenous TP53 in human ovarian cancer and complements its validated use for immunoprecipitation.