p53 Antibody Clone PAb 122 / TP53 Tumor Suppressor Antibody / C-Terminal Region [clone PAb 122] (V3510)

p53 Antibody Clone PAb 122 / TP53 Tumor Suppressor Antibody Clone PAb 122. Protein microarray specificity validation using a HuProt(TM) human protein array containing more than 19,000 full-length human proteins demonstrates strong selective binding of the p53 Antibody Clone PAb 122 to TP53. The ranked signal plot shows TP53 as the dominant target with a markedly elevated signal compared with other proteins on the array, indicating high specificity of clone PAb 122 for Tumor protein p53.
The Z-score represents the strength of antibody binding to each protein target detected using a fluorescently labeled secondary antibody. Z-scores are expressed in standard deviations above the mean signal across the array. Proteins are ranked by descending Z-score, and the S-score represents the difference between adjacent Z-scores in the ranked list. A high S-score indicates strong specificity of the antibody for its intended target relative to other proteins present on the array.

p53 Antibody Clone PAb 122 / TP53 Tumor Suppressor Antibody (clone PAb 122). Western blot analysis of human HEK293 cell lysate using p53 Antibody Clone PAb 122 detects a band at approximately 53 kDa, consistent with the predicted molecular weight of Tumor protein p53 / TP53. The result demonstrates detection of endogenous p53 protein in human 293 cells by western blot.