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Home >> Antibodies >> Anti-p53 Antibody

Anti-p53 Antibody [clone SPM589] (V9089)

  Catalog No Formulation Size Price (USD)  
Image V9089-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V9089-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V9089SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V9089IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Western blot testing of human HeLa cell lysate with anti-p53 antibody (clone SPM589). Expetected molecular weight ~53 kDa.
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-p53 antibody (clone SPM589).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name SPM589
Purity Protein G affinity chromatography
UniProt P04637
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-p53 antibody is available for research use only.
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Description

p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.

Application Notes

The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).

Storage

Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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