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Ki67 Antibody / MKI67 Proliferation Marker Antibody

Home >> Antibodies >> Ki67 Antibody / MKI67 Proliferation Marker Antibody

Ki67 Antibody / MKI67 Proliferation Marker Antibody

Ki67 Proliferation Marker Function and Cell Cycle Activity

Marker of proliferation Ki-67 (MKI67), commonly referred to as Ki67, is a nuclear protein associated with active cell division and is widely used as a marker of cellular proliferation. The Ki67 antibody is commonly used in research to evaluate growth activity in normal and cancer tissues, where its expression reflects the proportion of actively dividing cells. Because Ki67 is present during all active phases of the cell cycle and absent in quiescent cells, it serves as a reliable indicator of proliferative status.

Ki67 antibody, also known as MKI67 antibody or proliferation marker antibody, enables detection of nuclear protein expression across a wide range of tissues and experimental systems. Ki67 localization is confined to the nucleus during cell cycle progression, producing distinct staining patterns that correlate with cellular proliferation. In tumor biology, Ki67 expression is often evaluated alongside p53 tumor suppressor antibody to compare proliferative activity with regulatory checkpoint control.

Ki67 Antibody in Cell Cycle Regulation

Ki67 is expressed during G1, S, G2, and M phases of the cell cycle but is absent in resting (G0) cells, making it a highly specific marker of active proliferation. Ki67 antibody supports studies of cell cycle dynamics by identifying populations of cells that are actively progressing through division. This makes it a valuable tool for analyzing tissue growth, regeneration, and experimental perturbations affecting cell proliferation.

Ki67 Antibody in Cancer Biology

Ki67 is widely used in cancer research to assess tumor growth rate and biological aggressiveness. Elevated Ki67 expression is often associated with increased proliferation and more aggressive tumor behavior. Ki67 antibody enables analysis of these patterns across tumor types and is frequently used in conjunction with structural and lineage markers such as cytokeratin epithelial marker antibodies to better understand tumor composition and origin.

Ki67 Antibody in Tissue Growth and Regeneration

Beyond oncology, Ki67 plays an important role in developmental biology and tissue regeneration. Ki67 antibody enables identification of proliferating cells in normal tissues, supporting studies of stem cell activity, tissue repair, and growth regulation. Its expression provides insight into how tissues maintain and restore cellular populations.

Ki67 Antibody in Cellular Turnover and Stem Cell Biology

Ki67 is also used to study cellular turnover and stem cell dynamics, where distinguishing between proliferating and quiescent populations is critical. Ki67 antibody supports investigation of these processes by enabling detection of actively dividing cells across a wide range of biological contexts.

Due to its strong association with cell division, Ki67 remains one of the most widely used proliferation markers in biomedical research. Ki67 antibody provides a reliable tool for assessing cell cycle activity and understanding biological processes driven by cellular growth.

For additional antibody categories and targets, explore our antibody overview page.

Please find a sampling of the Ki67 antibodies available from NSJ Bioreagents below.

<p><strong data-start="147" data-end="300">Ki67 Antibody for IHC. Immunohistochemistry analysis of Ki67 / MKI67 expression in human tissue microarray (TMA) using Ki67 antibody clone <a href="../tds/ki-67-antibody-for-ihc-ki67-ihc-antibody-marker-of-proliferation-ki-67-msva-267m-v6097">MSVA-267M</a>.</strong> FFPE human tissue microarrays containing a broad range of normal and cancer tissues show nuclear HRP-DAB brown staining in proliferating cell populations, consistent with the expected nuclear localization of the Ki67 proliferation marker. In normal tissues, staining is largely confined to physiologic proliferative compartments including germinal centers, basal epithelial layers, and intestinal crypts, while most differentiated tissues exhibit minimal signal. In cancer tissues, many carcinomas demonstrate increased fractions of Ki67-positive nuclei, reflecting elevated cellular proliferation. The staining pattern aligns with established MKI67 expression profiles and supports the use of Ki67 antibody for tumor proliferation analysis and cancer biomarker research.</p>

Ki67 Antibody for IHC. Immunohistochemistry analysis of Ki67 / MKI67 expression in human tissue microarray (TMA) using Ki67 antibody clone MSVA-267M. FFPE human tissue microarrays containing a broad range of normal and cancer tissues show nuclear HRP-DAB brown staining in proliferating cell populations, consistent with the expected nuclear localization of the Ki67 proliferation marker. In normal tissues, staining is largely confined to physiologic proliferative compartments including germinal centers, basal epithelial layers, and intestinal crypts, while most differentiated tissues exhibit minimal signal. In cancer tissues, many carcinomas demonstrate increased fractions of Ki67-positive nuclei, reflecting elevated cellular proliferation. The staining pattern aligns with established MKI67 expression profiles and supports the use of Ki67 antibody for tumor proliferation analysis and cancer biomarker research.

Found all antibodies, displaying 1 to 10
Ki-67 Antibody / Marker of proliferation Ki-67 (clone MSVA-267M)
Catalog No : V6097
Applications : IHC
Reactivity : Human
Format : Purified
Ki-67 Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Ki-67 / MKI67 in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using mouse monoclonal antibody clone MSVA-267M. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates nuclear localization in proliferating cell populations, consistent with the role of Ki-67 as a proliferation marker. In normal tissue microarrays, staining is largely restricted to physiologic proliferative compartments, including germinal centers in lymphoid tissues, basal epithelial layers, and intestinal crypt cells, while most differentiated tissues show minimal to absent signal. Within tumor tissue microarrays, many carcinomas and other malignancies display elevated fractions of Ki-67-positive nuclei, reflecting increased cellular proliferation. Evaluation across large TMA panels enables direct comparison of MKI67 expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported Ki-67 expression profiles in the Human Protein Atlas and support its use as a marker of cell proliferation and tumor growth fraction.
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Ki-67 Antibody / MKI67 (clone 31K02)
Catalog No : FY12964
Applications : IHC, IF, ICC/IF
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Ki-67 Antibody Clone 31K02 / MKI67 Antibody Clone 31K02. Immunohistochemistry analysis of paraffin-embedded mouse spleen tissue using Ki-67 antibody clone 31K02. Strong nuclear brown staining is observed in numerous proliferating lymphoid cells within splenic white pulp regions, consistent with the known expression pattern of Ki-67 / MKI67 in actively dividing cells. Surrounding cells with lower proliferative activity show comparatively weaker staining. Heat-induced antigen retrieval was performed in EDTA buffer (pH 8.0) prior to staining. The tissue section was incubated with Ki-67 antibody clone 31K02 followed by peroxidase-conjugated goat anti-rabbit IgG secondary antibody, and signal was visualized using an HRP detection system with DAB chromogenic substrate.
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Ki-67 Antibody (clone MKI67/2466)
Catalog No : V7432
Applications : FACS, IF, IHC-P
Reactivity : Human
Format : Purified
Microvalidated
Ki-67 Antibody for IF (clone MKI67/2466) immunofluorescence staining of human HeLa cells. Human HeLa cells were stained with Ki-67 Antibody for IF (clone MKI67/2466). Green fluorescence highlights nuclear Ki-67 / MKI67 expression in proliferating cells, showing the characteristic punctate nuclear staining pattern associated with this cell cycle–regulated proliferation marker. Cell boundaries and actin-rich structures are visualized with Phalloidin (red-purple), allowing clear visualization of cellular morphology and confirming nuclear localization of the Ki-67 signal in actively cycling cells.
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Ki-67 Antibody (clone MKI67/2462)
Catalog No : V3864
Applications : FACS, IF, IHC-P, WB
Reactivity : Human
Format : Purified
Microvalidated
IHC testing of FFPE human skin stained with Ki67 antibody (MKI67/2462). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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Ki-67 Antibody (clone 5E12.)
Catalog No : RQ6529
Applications : IHC-P, IF, FACS
Reactivity : Human
Format : Antigen affinity purified
Ki-67 Antibody for IF Staining / Ki67 Staining Antibody (clone 5E12). Immunofluorescence analysis of FFPE human tonsil tissue using Ki-67 antibody for IF staining (clone 5E12). Strong nuclear fluorescence (green) is observed in numerous lymphoid cells within the germinal center region, consistent with the high proliferative activity typical of tonsillar lymphoid tissue. Ki-67 / MKI67 nuclear staining highlights actively cycling cells within the lymphoid follicle. Heat-induced epitope retrieval was performed by steaming tissue sections in pH6 citrate buffer for 20 minutes prior to immunofluorescence staining.
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Ki-67 antibody (clone 2179CT8.1.2.1)
Catalog No : F54499
Applications : IHC-P
Reactivity : Human
Format : Purified
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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Ki-67 Antibody (clone MKI67/2465)
Catalog No : V3866
Applications : FACS, IF, IHC-P
Reactivity : Human
Format : Purified
Microvalidated
Ki-67 Antibody - Protein Microarray Validated / MKI67 Microarray Validated Antibody (clone MKI67/2465). Immunohistochemistry analysis of FFPE human tonsil tissue using protein microarray validated Ki-67 antibody (clone MKI67/2465). Strong nuclear brown staining is observed in numerous proliferating lymphoid cells within the tonsillar germinal center, consistent with the known expression pattern of Ki-67 / MKI67 in actively dividing cells. The microarray validated antibody highlights proliferating lymphocytes while surrounding non-proliferating stromal and background cells show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM citrate buffer (pH6) for 10-20 minutes followed by cooling at room temperature prior to staining.
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Ki-67 Antibody (clone PMKI67-1)
Catalog No : V3871
Applications : IHC-P, FACS, IF
Reactivity : Human
Format : Purified
IHC testing of FFPE human tonsil stained with Ki67 antibody (clone PMKI67-1). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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