Ki-67 Antibody Clone 31K02 / MKI67 Antibody [clone 31K02] (FY12964)

Ki-67 Antibody Clone 31K02 / MKI67 Antibody Clone 31K02. Immunohistochemistry analysis of paraffin-embedded mouse spleen tissue using Ki-67 antibody clone 31K02. Strong nuclear brown staining is observed in numerous proliferating lymphoid cells within splenic white pulp regions, consistent with the known expression pattern of Ki-67 / MKI67 in actively dividing cells. Surrounding cells with lower proliferative activity show comparatively weaker staining. Heat-induced antigen retrieval was performed in EDTA buffer (pH 8.0) prior to staining. The tissue section was incubated with Ki-67 antibody clone 31K02 followed by peroxidase-conjugated goat anti-rabbit IgG secondary antibody, and signal was visualized using an HRP detection system with DAB chromogenic substrate.

Immunohistochemical staining of Ki67/MKI67 using anti-Ki-67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ki-67 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Immunohistochemical staining of Ki67/MKI67 using anti-Ki-67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ki-67 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Ki-67 Antibody Clone 31K02 / MKI67 Antibody Clone 31K02. Immunofluorescence analysis of paraffin-embedded human intestinal carcinoma tissue using Ki-67 antibody clone 31K02. Strong nuclear fluorescence (green) is observed in numerous proliferating tumor epithelial cells, consistent with the known nuclear localization of Ki-67 / MKI67 during active phases of the cell cycle. Non-proliferating surrounding cells show reduced fluorescence signal. Heat-induced antigen retrieval was performed in EDTA buffer (pH 8.0) prior to staining. The tissue section was incubated with Ki-67 antibody clone 31K02 followed by DyLight 488 conjugated goat anti-rabbit IgG secondary antibody, and fluorescence signal was visualized using appropriate fluorescence microscopy filter sets.

Immunofluorescent staining of Ki67/MKI67 using anti-Ki-67 antibody (green). Ki67/MKI67 was detected in an immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Ki-67 antibody overnight at 4oC. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.