Ki-67 Antibody for IF Staining|Ki67 Staining Antibody|RQ6529

	Catalog No	Formulation	Size	Price (USD)
	RQ6529	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

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Ki-67 Antibody for IF Staining / Ki67 Staining Antibody (clone 5E12). Immunofluorescence analysis of FFPE human tonsil tissue using Ki-67 antibody for IF staining (clone 5E12). Strong nuclear fluorescence (green) is observed in numerous lymphoid cells within the germinal center region, consistent with the high proliferative activity typical of tonsillar lymphoid tissue. Ki-67 / MKI67 nuclear staining highlights actively cycling cells within the lymphoid follicle. Heat-induced epitope retrieval was performed by steaming tissue sections in pH6 citrate buffer for 20 minutes prior to immunofluorescence staining.

Ki-67 Antibody for IF Staining / Ki67 Staining Antibody (clone 5E12). Immunofluorescence analysis of FFPE human A431 cells using Ki-67 antibody for IF staining (clone 5E12). Strong nuclear fluorescence (green) identifies proliferating cells expressing Ki-67 / MKI67, a nuclear marker associated with active phases of the cell cycle. Nuclei are counterstained with DAPI (blue), enabling visualization of total cell nuclei and facilitating comparison between Ki-67 positive proliferating cells and the broader cell population. Heat-induced epitope retrieval was performed by steaming sections in pH6 citrate buffer for 20 minutes prior to staining.

IHC staining of FFPE human liver cancer tissue with Ki-67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with Ki67 Staining Antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with Ki-67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human esophageal squamous carcinoma tissue with Ki-67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cervical cancer tissue with Ki67 Staining Antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of human Jurkat cells with Ki67 Staining Antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ki-67 antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b
Clone Name	5E12.
Purity	Affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P46013
Localization	Nuclear
Applications	Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This Ki-67 antibody is available for research use only.
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Description

Ki-67 antigen (MKI67) is a nuclear proliferation-associated protein encoded by the MKI67 gene and is widely used as a marker of actively cycling cells. Ki-67 Antibody for IF Staining / Ki67 Staining Antibody (clone 5E12) supports immunofluorescence staining approaches that allow researchers to visualize proliferating cells and analyze cell cycle activity using fluorescence microscopy. This IF-based staining method enables direct imaging of nuclear Ki-67 distribution within cultured cells and tissue sections.

Ki-67 antibody, also referred to as Ki67 antibody or MKI67 antibody in the literature, recognizes a nuclear protein expressed during the G1, S, G2, and mitotic phases of the cell cycle but largely absent from quiescent G0 cells. Because Ki-67 expression correlates closely with cellular proliferation, Ki-67 Antibody for IF Staining is widely used to identify actively dividing cells and to evaluate proliferative fractions in biological samples using immunofluorescence staining techniques.

Immunofluorescence staining with Ki-67 Antibody for IF Staining typically produces bright nuclear fluorescence in proliferating cells. During interphase, Ki-67 staining often appears as punctate or nucleolar-associated nuclear signal patterns. As cells progress through mitosis, Ki-67 redistributes across condensed chromosomes and perichromosomal regions, producing distinctive staining patterns that can be visualized by fluorescence microscopy. These changes in fluorescence staining patterns provide useful visual indicators of cell cycle progression and mitotic activity.

Because Ki-67 undergoes dynamic redistribution during mitosis, Ki-67 Antibody for IF Staining is frequently used in cell cycle imaging experiments designed to track proliferating cells and to study mitotic chromosome organization. Fluorescence staining of Ki-67 allows investigators to visualize nuclear architecture during different phases of the cell cycle and to distinguish proliferating cells from non-dividing populations within the same culture.

Ki-67 Antibody for IF Staining can also be incorporated into multicolor immunofluorescence staining experiments. In these applications, nuclear Ki-67 staining can be combined with cytoskeletal markers, membrane stains, or DNA dyes to enable simultaneous visualization of cell structure, nuclear morphology, and proliferative status. Such fluorescence staining strategies support detailed imaging of cell cycle dynamics, making Ki-67 Antibody for IF Staining (clone 5E12) a useful tool for fluorescence-based cell cycle imaging and proliferation studies.

Application Notes

Optimal dilution of the Ki-67 Antibody for IF Staining should be determined by the researcher.

Immunogen

An E. coli-derived human protein (amino acids K2860-I3256) was used as the immunogen for the Ki-67 antibody.

Storage

After reconstitution, the Ki-67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Ki67 antibody, MKI67 antibody, Antigen KI-67 antibody, Ki-67 proliferation marker antibody, Nuclear proliferation antigen Ki-67 antibody

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Ki-67 Antibody for IF Staining / Ki67 Staining Antibody [clone 5E12.] (RQ6529)

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