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Home >> Antibodies >> Ki67 Antibody

Ki67 Antibody [clone PMKI67-1] (V3871)

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  Catalog No Formulation Size Price (USD)  
Image V3871-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3871-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3871SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human tonsil stained with Ki67 antibody (clone PMKI67-1). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil stained with Ki67 antibody (clone PMKI67-1). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human HeLa cells with Ki67 antibody (green, clone PMKI67-1) and Phalloidin (red, membrane stain).
FACS testing of human HeLa cells with Ki67 antibody (blue, clone PMKI67-1) and isotype control (red). Cells were trypsinized and 2-4% PFA-fixed prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name PMKI67-1
Purity Protein G affinity chromatography
UniProt P46013
Localization Nuclear
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Ki67 antibody is available for research use only.
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Description

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.

Storage

Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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