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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Ki-67 Antibody Fluorescence Imaging / Ki67 IF Antibody [clone PMKI67-1] (V3871)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3871-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V3871-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V3871SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | PMKI67-1 |
| Purity | Protein G affinity chromatography |
| UniProt | P46013 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/10^6 cells in 0.1ml Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Ki67 antibody is available for research use only. |
|
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Ki-67 antigen (MKI67) is a nuclear proliferation-associated protein encoded by the MKI67 gene and is widely used as a marker of actively cycling cells. Ki-67 Antibody Fluorescence Imaging / Ki67 IF Antibody (clone PMKI67-1) enables visualization of proliferating cells using fluorescence imaging approaches that support high-resolution microscopy and subcellular localization analysis of Ki-67 within the nucleus. Fluorescence imaging with Ki-67 antibodies allows investigators to identify proliferating cells while examining nuclear architecture and cellular morphology in the same field of view.
Ki-67 antibody, also referred to as Ki67 antibody or MKI67 antibody in the literature, recognizes a nuclear protein that is present during the G1, S, G2, and mitotic phases of the cell cycle but absent in quiescent G0 cells. Because Ki-67 expression correlates closely with cellular proliferation, fluorescence imaging using Ki-67 antibodies is widely used to visualize proliferating cells in cultured cell models and tissue samples using fluorescence microscopy.
Ki-67 Antibody Fluorescence Imaging produces characteristic nuclear fluorescence patterns that reflect the subcellular distribution of Ki-67 during different phases of the cell cycle. During interphase, Ki-67 localizes within the nucleolus and surrounding nuclear regions, often appearing as punctate fluorescence signals. As cells enter mitosis, Ki-67 redistributes to perichromosomal regions surrounding condensed chromosomes, creating distinctive fluorescence patterns that can be visualized using high-resolution fluorescence microscopy.
High-resolution fluorescence imaging with Ki-67 antibodies allows researchers to examine subcellular localization of Ki-67 in relation to chromatin organization and nuclear structure. Using appropriate fluorescence microscopy systems, Ki-67 positive nuclei can be visualized at high spatial resolution, enabling detailed imaging of nuclear protein distribution and cell cycle-dependent localization patterns. These imaging approaches are particularly useful when studying proliferative cell populations in cell biology and cancer research.
Ki-67 Antibody Fluorescence Imaging can also be incorporated into multicolor fluorescence microscopy experiments. In such studies, Ki-67 nuclear fluorescence can be visualized together with cytoskeletal markers, membrane stains, or nuclear dyes, enabling simultaneous observation of cell morphology, nuclear organization, and proliferative status. Through high-resolution fluorescence microscopy and subcellular localization analysis, Ki-67 Antibody Fluorescence Imaging (clone PMKI67-1) supports detailed imaging of proliferating cells using fluorescence-based experimental workflows.
The stated application concentrations are suggested starting points. Titration of the Ki-67 Antibody Fluorescence Imaging may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.
Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Ki67 antibody, MKI67 antibody, Antigen KI-67 antibody, Ki-67 proliferation marker antibody, Nuclear proliferation antigen Ki-67 antibody
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