NSJ Bioreagents

Cytokeratins are a group of intermediate filaments that play a crucial role in maintaining the structural integrity of epithelial cells. Among the various subtypes of cytokeratins, Cytokeratin 17 stands out for its diverse functions and expression patterns. This protein can be identified in cells and tissue using a Ctyokeratin 17 antibody and is typically overexpressed in various types of cancers, including breast, prostate, and lung cancer, making it a potential biomarker for disease diagnosis and prognosis. Studies have shown that dysregulation of Cytokeratin 17 expression is associated with the development and progression of various diseases. In cancer, increased levels of Cytokeratin 17 have been linked to tumor cell proliferation, invasion, and metastasis, highlighting its role as a potential therapeutic target. Additionally, Cytokeratin 17 has been implicated in skin disorders, such as psoriasis and wound healing, further demonstrating its importance in disease pathogenesis. The unique properties of Cytokeratin 17 make it a valuable tool for understanding complex cellular processes and disease mechanisms. Researchers have utilized Cytokeratin 17 as a biomarker for cancer diagnosis and prognosis, as well as a target for developing novel therapeutic strategies. Furthermore, the study of Cytokeratin 17 in skin biology has provided insights into wound healing mechanisms and potential treatment options for skin disorders.

The Cytokeratin 17 antibody holds great potential for researchers looking to study cellular structure and function using techniques such as western blot, flow cytometry, immunofluorescence, immunohistochemistry, and ELISA.

Western blot is a common technique used to detect the presence of Cytokeratin 17 in cell lysates with a cytokeratin 17 antibody. By separating proteins based on their size and charge, researchers can identify the specific band corresponding to Cytokeratin 17. This technique provides valuable information about the expression level of Cytokeratin 17 in different cell types and under various conditions.

Flow cytometry is another powerful tool for analyzing Cytokeratin 17 expression at the single-cell level. By using a fluorescently labeled Cytokeratin 17 antibody, researchers can quantify the amount of Cytokeratin 17 present in individual cells and identify cell populations based on their cytokeratin 17 expression profile. This technique is particularly useful for studying heterogeneous cell populations and identifying rare cell types.

Immunofluorescence is a popular technique for visualizing the subcellular localization of Cytokeratin 17 within cells. Researchers can use a Cytokeratin 17 antibody to pinpoint the exact location of the protein within the cell and examine its distribution in different cellular compartments. This technique provides valuable insights into the role of Cytokeratin 17 in cell structure and function.

Immunohistochemistry is a key technique for studying Cytokeratin 17 expression in tissue samples. By using a Cytokeratin 17 antibody to label specific cells or tissues, researchers can visualize the distribution and abundance of Cytokeratin 17 in various tissues and organs. This technique is essential for understanding the role of Cytokeratin 17 in tissue development, homeostasis, and disease.

ELISA (enzyme-linked immunosorbent assay) is a highly sensitive technique for quantifying the amount of Cytokeratin 17 protein in biological samples. By using a Cytokeratin 17 antibody to capture and detect cytokeratin 17 molecules, researchers can accurately measure the concentration of the protein in blood, urine, or tissue samples. This technique is valuable for biomarker discovery and monitoring disease progression.

The Cytokeratin 17 antibody is a powerful tool for studying the expression and function of cytokeratin 17 in various cellular and tissue contexts. By harnessing the diverse techniques available, researchers can unravel the complex role of cytokeratin 17 in health and disease.