- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cytokeratin 17 Antibody for FACS / Keratin 17 Flow Cytometry Antibody [clone SPM560] (V9059)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V9059-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V9059-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V9059SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V9059IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | SPM560 |
| Purity | Protein G affinity chromatography |
| UniProt | Q04695 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 1-2ug/ml Flow Cytometry : 1-2ug/10^6 cells Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Cytokeratin 17 antibody is available for research use only. |
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Cytokeratin 17 (KRT17), also known as Keratin 17 or CK17, is a type I intermediate filament protein encoded by the KRT17 gene and expressed in epithelial tissues. Cytokeratin 17 contributes to the structural cytoskeleton of epithelial cells, forming part of the keratin intermediate filament network that supports cellular integrity and tissue architecture. Cytokeratin 17 Antibody for FACS (clone SPM560) recognizes the KRT17 protein and supports research applications focused on flow cytometry analysis of epithelial cell populations.
Flow cytometry provides a powerful approach for analyzing protein expression in large populations of cells at single-cell resolution. Because Cytokeratin 17 is an intracellular cytoskeletal protein, detection by flow cytometry typically involves fixation and permeabilization steps that allow antibodies to access intracellular targets. Using Cytokeratin 17 Antibody for FACS, researchers can evaluate CK17 expression in epithelial cells, tumor-derived cell lines, or dissociated tissue samples while simultaneously measuring additional cellular markers in multiparametric flow cytometry experiments.
Keratin intermediate filaments are assembled from type I and type II keratin proteins that polymerize into filamentous networks throughout the cytoplasm. These networks provide mechanical resilience and structural stability to epithelial cells. Cytokeratin 17 participates in this filament system by pairing with complementary keratin proteins to form intermediate filaments that reinforce epithelial cell architecture. Detection of KRT17 expression by flow cytometry can therefore help identify epithelial lineage cells within mixed cell populations or assess epithelial differentiation states at the single-cell level.
In cancer research, Cytokeratin 17 expression has been widely investigated as a marker associated with epithelial tumors. Flow cytometry analysis using Cytokeratin 17 Antibody for FACS allows researchers to quantify CK17-positive tumor cells, evaluate heterogeneity within tumor cell populations, and isolate epithelial-derived cells for downstream analysis. Flow cytometry detection of cytokeratin proteins is commonly used in studies examining circulating tumor cells, epithelial lineage markers, and epithelial cell enrichment from complex biological samples.
Because keratin expression patterns vary among epithelial cell types, cytokeratin profiling is frequently used to characterize epithelial differentiation and tissue origin. Flow cytometric detection of CK17 can complement other epithelial markers in multiparametric panels, enabling detailed characterization of epithelial and tumor cell populations.
Cytokeratin 17 Antibody for FACS (clone SPM560) is a mouse monoclonal antibody developed for research applications involving flow cytometry. Detection of Cytokeratin 17 using clone SPM560 supports studies examining epithelial cell populations, epithelial tumor biology, and intracellular keratin expression using flow cytometry-based analysis.
The optimal dilution of the Cytokeratin 17 Antibody for FACS for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
A cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.
Store the Cytokeratin 17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CK17 antibody, Keratin 17 antibody, KRT17 antibody, Cytokeratin-17 antibody
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