KRT17 Antibody SPM560 / Cytokeratin 17 Marker [clone SPM560] (V9059)

KRT17 Antibody SPM560 Staining of Human Skin Epithelial Appendages IHC. Immunohistochemistry of FFPE human skin using KRT17 Antibody clone SPM560. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min. Strong cytoplasmic staining is concentrated within an epithelial appendage, while the surrounding stromal tissue shows minimal staining, demonstrating localization of the Cytokeratin 17 marker to epithelial cells.

KRT17 Antibody SPM560 Detection in Human Skin Adnexal Structures IHC. Immunohistochemistry of FFPE human skin using KRT17 Antibody clone SPM560. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min. Cytoplasmic KRT17 staining highlights multiple epithelial adnexal structures against largely negative surrounding connective tissue, illustrating the restricted epithelial distribution of Cytokeratin 17 in skin.

Cytokeratin 17 Marker Staining Pattern in Human Skin with SPM560 IHC. Immunohistochemistry of FFPE human skin using KRT17 Antibody clone SPM560. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min. At higher magnification, intense cytoplasmic staining is visible throughout the KRT17-positive epithelial cell population, clearly demonstrating the filament-associated Cytokeratin 17 staining pattern.

KRT17 Antibody SPM560 Detection in HeLa Cervical Cancer Cells WB. Western blot analysis of human HeLa cervical cancer cell lysate using KRT17 Antibody clone SPM560. A strong, discrete immunoreactive band is detected at approximately 45-48 kDa, consistent with the expected molecular weight of Cytokeratin 17. The result demonstrates detection of endogenous KRT17 in an epithelial-derived human cancer cell line.

KRT17 Antibody SPM560 Staining of HeLa Cervical Cancer Cells FACS. Flow cytometry analysis of PFA-fixed human HeLa cervical cancer cells using KRT17 Antibody clone SPM560. The antibody-stained population (blue) shows a clear rightward fluorescence shift relative to the isotype control (red), demonstrating detection of intracellular Cytokeratin 17 in HeLa cells and supporting recognition of the KRT17 epithelial marker by clone SPM560.