Cytokeratin 17 Antibody E3 / KRT17 Epithelial Marker [clone E3] (V2176)

Cytokeratin 17 Antibody E3 in Human Cervical Carcinoma IHC. Immunohistochemistry staining of FFPE human cervical carcinoma tissue with Cytokeratin 17 Antibody clone E3. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at room temperature for 20 min. Strong brown cytoplasmic staining is present in malignant epithelial cells, with comparatively little staining in the surrounding stromal and inflammatory compartments. The selective tumor epithelial staining demonstrates detection of the KRT17 epithelial marker with the Keratin 17 Antibody.

Cytokeratin 17 Antibody E3 in Human Bladder Carcinoma IHC. Immunohistochemistry staining of FFPE human bladder carcinoma tissue with Cytokeratin 17 Antibody clone E3. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at room temperature for 20 min. Intense brown cytoplasmic staining is concentrated within the malignant epithelial compartment, contrasting with the relatively weak staining of the adjacent stromal tissue. This prominent epithelial localization supports detection of KRT17 with the Keratin 17 Antibody.

Cytokeratin 17 Antibody E3 Shows Tumor-Selective Bladder Carcinoma Staining IHC. Immunohistochemistry staining of FFPE human bladder carcinoma tissue with Cytokeratin 17 Antibody clone E3. Strong HRP-DAB brown cytoplasmic staining is visible throughout the malignant epithelial cells, while the adjacent stromal compartment shows minimal labeling. This independent field further demonstrates the strong epithelial tumor localization of the KRT17 epithelial marker and reinforces tissue detection with the Keratin 17 Antibody.

Cytokeratin 17 Antibody E3 in HeLa Cervical Cancer Cells WB. Western blot analysis of human HeLa cervical cancer cell lysate with Cytokeratin 17 Antibody clone E3. Lane 1: HeLa cell lysate. A strong, discrete immunoreactive band is detected at approximately 48 kDa, consistent with the expected molecular weight of KRT17. The predominance of the expected target band with little additional reactivity demonstrates recognition of endogenous Cytokeratin 17 in this cervical cancer cell model and supports western blot performance of the Keratin 17 Antibody.

Cytokeratin 17 Antibody E3 Detection of KRT17 in HeLa Cells WB. Western blot analysis of human HeLa cervical cancer cell lysate with Cytokeratin 17 Antibody clone E3. Lane 1: HeLa cell lysate. A prominent band is detected at approximately 48 kDa, corresponding closely to the predicted molecular weight of the Cytokeratin 17/KRT17 intermediate filament protein. The strong target-sized signal provides an independent western blot demonstration of endogenous KRT17 detection and reinforces the specificity profile of the Keratin 17 Antibody.

Purified Cytokeratin 17 Antibody E3 SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free Cytokeratin 17 Antibody clone E3 under non-reducing (NR) and reducing (R) conditions. The non-reduced preparation contains predominantly intact immunoglobulin near 150 kDa, while reduction produces the expected antibody heavy and light chain components at approximately 50 kDa and 25 kDa. The electrophoretic profile supports the integrity and purity of the purified Keratin 17 Antibody preparation.