- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cytokeratin 17 Antibody [clone E3] (V2176)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V2176-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 539 | |
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V2176-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 249 | |
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V2176SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 | |
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V2176IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 539 |
| Species Reactivity | Human, Rat |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | E3 |
| Purity | Protein G affinity chromatography |
| Buffer | 1X PBS, pH 7.4 |
| Gene ID | 3872 |
| Localization | Cytoplasmic |
| Applications | Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Cytokeratin 17 antibody is available for research use only. |
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Cytokeratin 17 antibody clone E3 is a monoclonal antibody specific for cytokeratin 17, a type I intermediate filament protein expressed in epithelial cells under conditions of proliferation, repair, or neoplasia. Cytokeratin 17 is normally found in basal epithelial cells of certain tissues, including hair follicles, nail beds, and glandular ducts. It is strongly induced during wound healing and is frequently upregulated in a wide variety of carcinomas. NSJ Bioreagents provides Cytokeratin 17 antibody clone E3 to support research into epithelial regeneration, cancer biology, and diagnostic pathology.
In immunohistochemistry, cytokeratin 17 antibody clone E3 produces cytoplasmic staining in reactive and proliferative epithelial cells. It is often used to distinguish basal-like breast carcinomas and other high-grade tumors, where cytokeratin 17 serves as a marker of aggressive tumor behavior. In cervical pathology, cytokeratin 17 expression is associated with squamous intraepithelial lesions and invasive squamous carcinomas, making clone E3 a helpful diagnostic aid.
In dermatology research, cytokeratin 17 antibody clone E3 highlights keratinocytes involved in wound healing, where cytokeratin 17 supports migration and repair of epithelial surfaces. This makes the antibody valuable in experimental wound models and in studies investigating regenerative therapies. Cytokeratin 17 is also expressed in psoriasis and other hyperproliferative skin disorders, where its detection reflects underlying alterations in keratinocyte biology.
In cancer research, cytokeratin 17 antibody clone E3 has been used to study tumor progression and prognosis. Its overexpression is associated with reduced survival in several epithelial cancers, underscoring its importance as a prognostic biomarker. Researchers employ this antibody to track cytokeratin 17 expression across tumor types, including cervical, lung, pancreatic, and head and neck carcinomas.
Technically, cytokeratin 17 antibody clone E3 has been validated for immunohistochemistry, immunofluorescence, and western blotting. It provides clear, reproducible signals in both paraffin-embedded and frozen tissues. Its extensive publication history demonstrates its reliability across diverse applications. Alternate names include CK17 antibody, keratin 17 antibody, and keratin type I cytoskeletal 17 antibody.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
The cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.
Store the Cytokeratin 17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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