Cytokeratin 17 Antibody | KRT17 Epithelial Marker

	Catalog No	Formulation	Size	Price (USD)
	V2176-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V2176-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V2176SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation
	V2176IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	559
Formulation

Cytokeratin 17 Antibody E3 Staining of Human Cervical Carcinoma IHC. Immunohistochemistry of FFPE human cervical carcinoma tissue using Cytokeratin 17 Antibody clone E3. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris buffer containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at room temperature for 20 min. Strong cytoplasmic staining is observed in malignant epithelial cells, highlighting KRT17 expression within the cervical carcinoma.

Cytokeratin 17 Antibody E3 Staining of Human Bladder Carcinoma IHC. Immunohistochemistry of FFPE human bladder carcinoma tissue using Cytokeratin 17 Antibody clone E3. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris buffer containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at room temperature for 20 min. Strong cytoplasmic staining is observed in malignant epithelial cells, demonstrating detection of the KRT17 epithelial marker in bladder carcinoma.

Cytokeratin 17 Antibody E3 Detection in HeLa Cervical Cancer Cells WB. Western blot analysis of human HeLa cervical cancer cell lysate using Cytokeratin 17 Antibody clone E3. A strong immunoreactive band is detected at approximately 48 kDa, consistent with the expected molecular weight of KRT17. This result demonstrates recognition of endogenous Cytokeratin 17 in HeLa cells by the E3 monoclonal antibody.

Cytokeratin 17 Antibody Clone E3 western blot analysis of human HeLa cell lysate. Western blot detection using Cytokeratin 17 Antibody Clone E3 demonstrates a clear immunoreactive band in human HeLa cell lysate. Lane 1: human HeLa cell lysate. A band is detected at approximately 48 kDa, consistent with the predicted molecular weight of Cytokeratin 17 (CK17 / KRT17), an epithelial intermediate filament protein that contributes to the structural cytoskeleton of epithelial cells.

SDS-PAGE Analysis of Purified Cytokeratin 17 Antibody E3. SDS-PAGE analysis of purified, BSA-free Cytokeratin 17 Antibody clone E3 under reducing conditions. The antibody resolves into the expected immunoglobulin heavy and light chain components, supporting the integrity and purity of the purified mouse monoclonal antibody preparation.

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Cytokeratin 17 Antibody E3 Staining of Human Cervical Carcinoma IHC. Immunohistochemistry of FFPE human cervical carcinoma tissue using Cytokeratin 17 Antibody clone E3. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris buffer containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at room temperature for 20 min. Strong cytoplasmic staining is observed in malignant epithelial cells, highlighting KRT17 expression within the cervical carcinoma.

Cytokeratin 17 Antibody E3 Staining of Human Bladder Carcinoma IHC. Immunohistochemistry of FFPE human bladder carcinoma tissue using Cytokeratin 17 Antibody clone E3. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris buffer containing 1 mM EDTA, pH 9, for 10-20 min followed by cooling at room temperature for 20 min. Strong cytoplasmic staining is observed in malignant epithelial cells, demonstrating detection of the KRT17 epithelial marker in bladder carcinoma.

KRT17 Epithelial Marker Detection in Human Bladder Carcinoma with Clone E3 IHC. Immunohistochemistry of FFPE human bladder carcinoma tissue using Cytokeratin 17 Antibody clone E3. Strong HRP-DAB brown cytoplasmic staining is observed in malignant epithelial cells, while adjacent stromal cells show minimal staining. The staining pattern highlights KRT17 expression in the epithelial tumor compartment and supports use of clone E3 for detection of the Cytokeratin 17 epithelial marker.

Cytokeratin 17 Antibody E3 Detection in HeLa Cervical Cancer Cells WB. Western blot analysis of human HeLa cervical cancer cell lysate using Cytokeratin 17 Antibody clone E3. A strong immunoreactive band is detected at approximately 48 kDa, consistent with the expected molecular weight of KRT17. This result demonstrates recognition of endogenous Cytokeratin 17 in HeLa cells by the E3 monoclonal antibody.

Cytokeratin 17 Antibody Clone E3 western blot analysis of human HeLa cell lysate. Western blot detection using Cytokeratin 17 Antibody Clone E3 demonstrates a clear immunoreactive band in human HeLa cell lysate. Lane 1: human HeLa cell lysate. A band is detected at approximately 48 kDa, consistent with the predicted molecular weight of Cytokeratin 17 (CK17 / KRT17), an epithelial intermediate filament protein that contributes to the structural cytoskeleton of epithelial cells.

SDS-PAGE Analysis of Purified Cytokeratin 17 Antibody E3. SDS-PAGE analysis of purified, BSA-free Cytokeratin 17 Antibody clone E3 under reducing conditions. The antibody resolves into the expected immunoglobulin heavy and light chain components, supporting the integrity and purity of the purified mouse monoclonal antibody preparation.

Species Reactivity	Human, Rat
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	E3
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	3872
Localization	Cytoplasmic
Applications	Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This Cytokeratin 17 Antibody E3 / KRT17 Epithelial Marker is available for research use only.
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Description

Cytokeratin 17 Antibody / KRT17 Epithelial Marker detects keratin 17 (KRT17), also known as cytokeratin 17, CK17 or K17, a type I intermediate filament protein expressed in selected epithelial cell populations. Keratin intermediate filaments are major components of the epithelial cytoskeleton, where they contribute to mechanical stability, cellular architecture and resistance to physical stress. KRT17 has a characteristic distribution in basal epithelial compartments and specialized epithelial structures, making it an important marker for studying epithelial identity, differentiation and tissue organization.

KRT17 forms intermediate filament networks through association with type II keratin partners, particularly keratin 5 and keratin 6. These cytoplasmic filament systems provide structural support while participating in dynamic changes associated with epithelial growth and differentiation. In normal tissues, Cytokeratin 17 is found in selected basal and myoepithelial cell populations and in structures such as hair follicles and other appendageal epithelia. Its relatively restricted expression compared with broadly distributed epithelial keratins makes a Cytokeratin 17 Antibody useful for identifying KRT17-positive populations and investigating epithelial lineage.

Cytokeratin 17 is also responsive to changes in epithelial cell state. KRT17 expression can increase during wound healing, tissue regeneration and other conditions involving epithelial activation or hyperproliferation. Beyond its structural contribution to intermediate filaments, KRT17 has been linked to cellular processes associated with growth, stress responses and inflammatory signaling. These characteristics have expanded interest in the KRT17 epithelial marker as a research target for understanding how epithelial cells adapt during tissue injury, remodeling and disease.

Altered KRT17 expression is particularly relevant to cancer biology. Cytokeratin 17 has been investigated in a variety of epithelial malignancies, including squamous cell carcinomas and tumors of the breast, lung, pancreas and other tissues. Its expression can help define basal or squamous differentiation patterns within heterogeneous tumors and is frequently studied alongside other cytokeratins and epithelial markers. KRT17 expression has also been associated with aggressive tumor phenotypes in several cancer types, supporting continued investigation of its relationship to tumor cell proliferation, differentiation, invasion and disease progression.

Clone E3 is a well-established mouse monoclonal antibody directed against Cytokeratin 17. The antibody was generated against a cytoskeletal preparation from rat colon epithelium and recognizes the KRT17 intermediate filament protein. Its established use in Cytokeratin 17 research makes clone E3 a useful reagent for investigating epithelial differentiation, cytoskeletal organization and changes in KRT17 expression associated with tumor biology.

Researchers studying KRT17 expression, epithelial differentiation and tumor biology can explore additional reagents on our Cytokeratin 17 Antibody / KRT17 Epithelial Marker Antibody page. NSJ Bioreagents provides Cytokeratin 17 antibodies for research into epithelial biology and cancer-associated changes in keratin expression. A Cytokeratin 17 Antibody provides a research tool for detecting KRT17 and studying this intermediate filament protein in epithelial differentiation, tissue remodeling and cancer biology.

Researchers studying KRT17 expression, epithelial differentiation and tumor biology can explore additional reagents on our Cytokeratin 17 Antibody / KRT17 Epithelial Marker Antibody page.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cytokeratin 17 Antibody E3 / KRT17 Epithelial Marker to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.

Storage

Store the Cytokeratin 17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CK17 antibody, Keratin 17 antibody, KRT17 antibody, Cytokeratin-17 antibody

Cytokeratin 17 Antibody E3 / KRT17 Epithelial Marker [clone E3] (V2176)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents