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Phospho Antibody / Antibodies

Home >> Antibodies >> Phospho Antibody / Antibodies

Phospho Antibody / Antibodies

Phospho antibodies available from NSJ Bioreagents:
Found all antibodies, displaying 291 to 300
Phospho-eIF4EBP1 (pThr46) Antibody / Eukaryotic translation initiation factor 4E binding protein 1 (clone 32E52)
Catalog No : FY12420
Applications : WB, IHC
Reactivity : Human, Mouse
Format : Liquid
Recrabbitmono
Western blot analysis of 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) using anti-Phospho-eIF4EBP1 (pThr46) antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) at approximately 18 kDa. The expected molecular weight of 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) is at 18 kDa.
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Phospho-Chk1 (pSer280) Antibody / Checkpoint kinase 1 (clone 31C46)
Catalog No : FY12496
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-Chk1 (pS280) expression in 293T treated with Calyculin A cell lysate, using Phospho-Chk1 (pSer280) antibody.
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Phospho-Beta Catenin (pThr41/pSer45) Antibody (clone 32C69)
Catalog No : FY12499
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
All lanes use the Phospho-Beta Catenin (pThr41/pSer45) antibody at 1:1000 dilution for 1 hour at room temperature.
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Phospho-CRMP2 (pThr514) Antibody / Collapsin response mediator protein 2 (clone 32D10)
Catalog No : FY12539
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
All lanes use the Phospho-CRMP2 (pThr514) antibody at 1:6000 dilution for 1 hour at room temperature. Phospho-CRMP2 (~62 kDa predicted) was detected as a major band at ~63-65 kDa, consistent with phosphorylation at GSK3β/CDK5-regulated residues Thr509, Thr514, and Ser522, which slightly retard electrophoretic mobility relative to total CRMP2.
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Phospho-c-Jun/JunD (pSer73/pSer100) Antibody (clone 30J61)
Catalog No : FY12568
Applications : WB, IHC, IP
Reactivity : Human, Mouse
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-c-Jun (S73)+JunD (S100) expression in mouse RAW 264.7 cell lysate using Phospho-c-Jun/JunD (Ser73/Ser100) antibody. Phospho-c-Jun/JunD (pSer73/pSer100) was detected in RAW264.7 lysate as a ~38-45 kDa doublet, consistent with JNK/MAPK-dependent multi-site phosphorylation of c-Jun and JunD that produces reduced-mobility bands on SDS-PAGE.
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Phospho-FAK (pTyr576/pTyr577) Antibody / Focal Adhesion Kinase 1 (clone 32P46)
Catalog No : FY12572
Applications : WB
Reactivity : Human, Mouse
Format : Liquid
Recrabbitmono
Mouse kidney tissue lysate tested with Phospho-FAK (pTyr576/pTyr577) antibody at 1:2000 dilution for 1 hour at room temperature. Predicted molecular weight ~120 kDa.
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Phospho-PKR (pThr451) Antibody / Protein kinase R / EIF2AK2 (clone 32E50)
Catalog No : FY12582
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Phospho-Vimentin (pSer39) Antibody / VIM (clone 31V35)
Catalog No : FY12596
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-Vimentin (pS39) expression in HeLa treated with Calyculin A cell lysate using Phospho-Vimentin (pSer39) antibody. Predicted molecular weight ~53-58 kDa.
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Phospho-Nucleophosmin (pSer125) Antibody / NPM1 (clone 32N71)
Catalog No : FY13010
Applications : WB, IHC
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Western blot testing of human samples using the Phospho-Nucleophosmin (pSer125) antibody at 1:3000 dilution for 1 hour at room temperature. A predominant band is observed at ~35 kDa, consistent with phosphorylated NPM1 migrating slightly above its predicted 33 kDa. An additional ~26 kDa species is detected, consistent with a Ser125-containing proteolytic fragment of NPM1 that appears in some cell lysates.
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Phospho-Protein Kinase D2 (pSer876) Antibody / PRKD2 (clone 32P58)
Catalog No : FY13086
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono