- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Phospho-PKC alpha (Thr497) Antibody / Protein kinase C alpha [clone 32P00] (FY12403)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12403 | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA | 100 ul | 439 |
Phospho-PKC alpha Thr497 antibody recognizes protein kinase C alpha phosphorylated at threonine 497, an activation site essential for catalytic function. PKC alpha is a member of the conventional protein kinase C family that functions as a serine threonine kinase. It is activated by calcium, diacylglycerol, and phospholipids, and it regulates processes including cell proliferation, differentiation, apoptosis, and migration. Phosphorylation at Thr497 stabilizes the kinase in an active conformation and promotes downstream substrate phosphorylation.
Phospho-PKC alpha Thr497 antibody is widely applied in signaling and phosphorylation research. By distinguishing the phosphorylated active form from inactive PKC alpha, it enables precise monitoring of pathway activation. This is particularly relevant in studies of cancer, cardiovascular disease, and neuronal signaling where PKC alpha activity is tightly regulated.
The antibody is validated for use in western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho PKC alpha Thr497 antibody detects phosphorylated isoforms and allows quantification of activation under various stimuli. In immunohistochemistry, it highlights phosphorylation dependent localization within tissues, while immunofluorescence provides single cell resolution of active PKC alpha.
PKC alpha has been implicated in cancer progression, where dysregulated activation contributes to survival, proliferation, and metastasis. Monitoring Thr497 phosphorylation with phospho specific antibodies provides mechanistic insight into tumor biology and therapeutic targeting. In cardiovascular research, PKC alpha activity is linked to hypertrophy, contractility, and vascular remodeling. In the nervous system, PKC alpha influences synaptic plasticity and long term potentiation.
Phospho-PKC alpha Thr497 antibody offered by NSJ Bioreagents provides strong specificity for activated PKC alpha, supporting studies across oncology, cardiology, and neuroscience. It is a critical reagent for examining phosphorylation dependent signaling in health and disease.
Optimal dilution of the Phospho-PKC alpha (Thr497) antibody should be determined by the researcher.
A synthesized peptide derived from human Phospho-PKC alpha (T497) was used as the immunogen for the Phospho-PKC alpha (Thr497) antibody.
Store the Phospho-PKC alpha (Thr497) antibody at -20oC.
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