- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Phospho-4EBP1 (pThr46) Antibody / Early Translation Control Marker [clone 32E52] (FY12420)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12420 | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA | 100 ul | 449 |
|
| Availability | 2-3 weeks |
| Species Reactivity | Human, Mouse |
| Format | Liquid |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | 32E52 |
| Purity | Affinity-chromatography |
| Buffer | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| UniProt | Q13541 , Q13542 , O60516 |
| Applications | Western Blot : 1:500-1:2000 Immunohistochemistry : 1:50-1:200 |
| Limitations | This Phospho-4EBP1 (pThr46) Antibody / Early Translation Control Marker is available for research use only. |
|
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Phospho-eIF4EBP1 (pThr46) antibody detects eukaryotic translation initiation factor 4E binding protein 1 when phosphorylated at threonine 46. EIF4EBP1 is encoded by the EIF4EBP1 gene and is part of a small family of translational repressors that also includes EIF4EBP2 and EIF4EBP3. These proteins function as regulators of cap dependent translation by binding to eIF4E and preventing assembly of the translation initiation complex. Phosphorylation of EIF4EBP1 at Thr46 and other regulatory sites causes its release from eIF4E, thereby promoting translation initiation.
Phospho-eIF4EBP1 (pThr46) antibody is widely applied in studies of mTOR signaling, translation control, and cancer biology. The mTOR pathway is a central regulator of cell growth, proliferation, and metabolism, and EIF4EBP1 phosphorylation is one of its key downstream events. By specifically detecting EIF4EBP1 phosphorylated at Thr46, this antibody provides a reliable marker of pathway activation and translational regulation.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-eIF4EBP1 (Thr46) antibody identifies phosphorylated protein bands distinct from non phosphorylated forms, allowing quantification of activation states. Immunohistochemistry provides tissue level mapping of phosphorylation patterns, while immunofluorescence reveals subcellular localization of activated EIF4EBP1. These applications make the antibody valuable for monitoring mTOR activity across experimental models.
EIF4EBP1 phosphorylation at Thr46 is conserved among family members, and antibodies raised against this site may also detect EIF4EBP2 at Thr46 and EIF4EBP3 at Thr32. This cross reactivity can be useful in capturing broader regulation of translation initiation but should be considered in experimental design. By applying Phospho-eIF4EBP1 (pThr46) antibody, researchers can evaluate translational control across multiple EIF4EBP proteins.
Dysregulation of EIF4EBP1 phosphorylation is common in cancers, where hyperactivation of mTOR signaling drives uncontrolled growth and survival. Detection of phospho EIF4EBP1 is frequently used as a biomarker of therapeutic response to mTOR inhibitors. Beyond oncology, mTOR EIF4EBP1 signaling regulates metabolism, neuronal plasticity, and immune responses, expanding the relevance of this antibody to diverse research fields.
Phospho-eIF4EBP1 (pThr46) antibody provided by NSJ Bioreagents delivers reliable specificity for phosphorylated EIF4EBP1, enabling accurate monitoring of mTOR activity and translational regulation in health and disease. This antibody is part of our full phospho antibody collection which can be explored for additional phosphorylation-specific targets and pathway markers.
Optimal dilution of the Phospho-4EBP1 (pThr46) Antibody / Early Translation Control Marker should be determined by the researcher.
A synthesized peptide derived from human Phospho-eIF4EBP1/2/3 (T46+T46+T32) was used as the immunogen for the Phospho-4EBP1 (pThr46) Antibody.
Store the Phospho-4EBP1 (pThr46) Antibody at -20oC.
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