Phospho-mTOR (pSer2448) Antibody / mTOR Signaling and Translation Control Marker [clone IFF-13] (RQ8814)

Phospho-mTOR (pSer2448) Antibody 4T1 WB. Western blot analysis of mouse 4T1 cell lysates including untreated, LPS-stimulated, and drug-treated conditions using phospho-mTOR antibody detecting mTOR phosphorylated at Ser2448, clone IFF-13. Lane 1: untreated, Lane 2: LPS-stimulated, Lane 3-5: increasing drug doses, Lane 6: positive control drug. A band is detected at approximately 289 kDa, consistent with the predicted molecular weight of mTOR. Phospho-mTOR levels vary across treatment conditions, reflecting modulation of mTOR pathway activity. Total mTOR and Actin are shown as controls.

Phospho-mTOR Antibody HEK293 WB. Western blot analysis of human HEK293 cell lysate using phospho-mTOR antibody detecting mTOR phosphorylated at Ser2448, clone IFF-13. A band is detected at approximately 250-290 kDa, consistent with the predicted molecular weight of mTOR. The signal supports detection of phosphorylated mTOR in HEK293 cells, a commonly used model for pathway signaling studies.

Phospho-mTOR Antibody Breast Cancer IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue stained with phospho-mTOR antibody detecting mTOR phosphorylated at Ser2448, clone IFF-13. Tumor epithelial cells show cytoplasmic HRP-DAB brown staining with moderate intensity, consistent with activation of mTOR signaling and protein synthesis pathways within malignant cell populations. Surrounding stromal cells display lower staining, while nuclei are counterstained blue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.