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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for c-Jun

c-Jun Antibody / JUN

Catalog No : RQ7978
Applications : WB, IHC-P, IF
Reactivity : Human
Format : Antigen affinity purified
JUN Antibody Laryngeal Squamous Cell Carcinoma IHC. Immunohistochemical staining of FFPE human laryngeal squamous cell carcinoma tissue using JUN Antibody demonstrates predominantly nuclear HRP-DAB brown staining within malignant squamous epithelial cell populations. The staining pattern is consistent with expression of JUN (c-Jun), a key component of the AP-1 transcription factor complex that regulates gene expression involved in cellular proliferation, differentiation, stress responses, and tissue remodeling. Nuclear localization of JUN is consistent with its established role as a DNA-binding transcription factor that mediates signal-dependent transcriptional regulation and cellular adaptation. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.
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c-Jun Antibody / JUN

Catalog No : RQ7005
Applications : WB, IHC-P, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
c-Jun Antibody A431 Cell IF. Immunofluorescent staining of FFPE human A431 cells using c-Jun Antibody (green) and Beta Tubulin Antibody (red) demonstrates prominent nuclear localization of c-Jun together with cytoplasmic microtubule staining of Beta Tubulin. The nuclear staining pattern is consistent with the function of c-Jun (JUN) as a DNA-binding transcription factor that regulates gene expression downstream of AP-1 signaling pathways. c-Jun plays important roles in transcriptional regulation, cellular differentiation, stress responses, proliferation, and signal transduction. Beta Tubulin staining highlights the cellular cytoskeleton, providing structural context for the nuclear localization of c-Jun. HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes prior to staining.
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c-Jun Antibody / JUN / Zebrafish

Catalog No : RZ1378
Applications : WB, IF
Reactivity : Zebrafish
Format : Antigen affinity purified
Zebrafish c-Jun Antibody Embryo IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish c-Jun Antibody / JUN Antibody demonstrates distinct green fluorescence within developing embryonic and neural-associated tissue structures, consistent with expression of c-Jun (JUN), a member of the AP-1 family of transcription factors. c-Jun functions as a key regulator of gene expression involved in embryonic development, cellular differentiation, stress responses, and tissue regeneration. The staining pattern highlights regions of active developmental signaling and transcriptional regulation within the embryo, while DAPI (blue) counterstains cell nuclei. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 5 µg/ml primary antibody, followed by DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1:500 dilution for 30 minutes at 37°C.
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c-Jun Antibody / JUN (clone CAO-10)

Catalog No : RQ5122
Applications : WB
Reactivity : Human, Mouse
Format : Purified
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JUN Antibody WB. Western blot testing of mouse NIH3T3 cell lysate with JUN antibody. Predicted molecular weight ~36 kDa, commonly observed at 36-43 kDa.
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Phospho-JUN antibody (pS63) (clone IGA-10)

Catalog No : RQ5123
Applications : WB
Reactivity : Human, Mouse
Format : Purified
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Phospho-c-Jun Antibody NIH3T3 WB. Western blot analysis of mouse NIH3T3 cell lysates untreated (-) or treated (+) with anisomycin using phospho-c-Jun antibody detecting c-Jun phosphorylated at Ser63, clone IGA-10. A band is observed at approximately 36 kDa, consistent with the predicted molecular weight of c-Jun. Signal intensity is increased in the anisomycin-treated sample, consistent with activation of JNK-mediated stress signaling.
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Phospho-c-Jun/JunD (pSer73/pSer100) Antibody (clone 30J61)

Catalog No : FY12568
Applications : WB, IHC, IP
Reactivity : Human, Mouse
Format : Liquid
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Western blot analysis of Phospho-c-Jun (S73)+JunD (S100) expression in mouse RAW 264.7 cell lysate using Phospho-c-Jun/JunD (Ser73/Ser100) antibody. Phospho-c-Jun/JunD (pSer73/pSer100) was detected in RAW264.7 lysate as a ~38-45 kDa doublet, consistent with JNK/MAPK-dependent multi-site phosphorylation of c-Jun and JunD that produces reduced-mobility bands on SDS-PAGE.
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JNK2 Antibody / MAPK9

Catalog No : R32105
Applications : WB, IF, FACS
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescence analysis of JNK2/MAPK9 expression in HeLa cells. Human HeLa cells were fixed and stained with anti-JNK2/MAPK9 antibody, followed by Fluoro488-conjugated goat anti-rabbit IgG secondary antibody (green). Nuclei were counterstained with DAPI (blue). JNK2/MAPK9 signal is observed predominantly in the cytoplasm with variable perinuclear distribution, consistent with stress-activated MAPK localization patterns.
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MEK7 Antibody / MAP2K7 / MKK7

Catalog No : R31800
Applications : WB, IHC-P, IF, FACS
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human PC-3 cells with MKK7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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MEK4 Antibody / MKK4 / MAP2K4

Catalog No : F54701
Applications : IHC-P, WB
Reactivity : Human, Mouse, Zebrafish
Format : Purified
Western blot testing of human Y79 cell lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
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JIP1 Antibody / MAPK8IP1

Catalog No : F55015
Applications : IF, FACS, IHC-P, WB
Reactivity : Human
Format : Purified
Western blot testing of human 1) brain and 2) MCF7 cell lysate with MAPK8IP1 antibody. Expected molecular weight: 78-110 kDa.
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