JNK2 Antibody / c-Jun N-terminal kinase 2 / MAPK9 (R32105)

Immunofluorescence analysis of JNK2/MAPK9 expression in HeLa cells. Human HeLa cells were fixed and stained with anti-JNK2/MAPK9 antibody, followed by Fluoro488-conjugated goat anti-rabbit IgG secondary antibody (green). Nuclei were counterstained with DAPI (blue). JNK2/MAPK9 signal is observed predominantly in the cytoplasm with variable perinuclear distribution, consistent with stress-activated MAPK localization patterns.

Western blot analysis of JNK2/MAPK9 expression. Whole cell lysates or tissue lysates from human SiHa cells (lane 1), human U251 cells (lane 2), human Caco-2 cells (lane 3), human A549 cells (lane 4), rat brain tissue (lane 5), rat C6 cells (lane 6), mouse brain tissue (lane 7), and mouse Neuro-2a cells (lane 8) were separated by SDS-PAGE and probed with anti-JNK2/MAPK9 antibody. A specific band is detected at the predicted molecular weight of approximately 48 kDa, consistent with c-Jun N-terminal kinase 2 (MAPK9). In several samples, a closely migrating doublet is observed around the predicted molecular weight, which is consistent with reported JNK2 isoforms and/or differential phosphorylation states commonly seen for stress-activated MAP kinases.

Flow cytometry analysis of fixed and permeabilized human HEL cells with JNK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JNK2 antibody.

Flow cytometry analysis of fixed and permeabilized mouse HEPA1-6 cells with JNK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JNK2 antibody.

Flow cytometry analysis of fixed and permeabilized rat RH35 cells with JNK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JNK2 antibody.