c-Jun Antibody / Transcriptional Regulation Protein Antibody (RQ7005)

c-Jun Antibody A431 Cell IF. Immunofluorescent staining of FFPE human A431 cells using c-Jun Antibody (green) and Beta Tubulin Antibody (red) demonstrates prominent nuclear localization of c-Jun together with cytoplasmic microtubule staining of Beta Tubulin. The nuclear staining pattern is consistent with the function of c-Jun (JUN) as a DNA-binding transcription factor that regulates gene expression downstream of AP-1 signaling pathways. c-Jun plays important roles in transcriptional regulation, cellular differentiation, stress responses, proliferation, and signal transduction. Beta Tubulin staining highlights the cellular cytoskeleton, providing structural context for the nuclear localization of c-Jun. HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes prior to staining.

c-Jun Antibody Renal Pelvis Squamous Metaplasia IHC. Immunohistochemical staining of FFPE human renal pelvis squamous metaplasia tissue using c-Jun Antibody demonstrates predominantly nuclear HRP-DAB brown staining within metaplastic epithelial cell populations. The staining pattern is consistent with expression of c-Jun (JUN), a DNA-binding transcription factor that functions as a key regulator of gene expression downstream of AP-1 signaling pathways. As a transcriptional regulation protein, c-Jun participates in cellular differentiation, stress responses, tissue remodeling, and signal transduction processes. Nuclear localization of c-Jun supports its role in controlling transcriptional programs associated with epithelial adaptation and phenotypic change. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.

c-Jun Antibody Renal Cell Carcinoma IHC. Immunohistochemical staining of FFPE human renal cell carcinoma tissue using c-Jun Antibody demonstrates prominent nuclear HRP-DAB brown staining throughout neoplastic cell populations. The staining pattern is consistent with expression of c-Jun (JUN), a DNA-binding transcription factor and key regulator of gene expression downstream of AP-1 signaling pathways. As a transcriptional regulation protein, c-Jun influences cellular proliferation, differentiation, stress responses, and tumor-associated signaling networks. The predominantly nuclear localization observed in tumor cells is consistent with the established role of c-Jun in controlling transcriptional programs that govern cellular adaptation and gene expression. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.

c-Jun Antibody Multi-Cell Line Western Blot. Western blot analysis of human HeLa (lane 1), K562 (lane 2), Jurkat (lane 3), and U-87 MG (lane 4) cell lysates using c-Jun Antibody demonstrates bands at approximately 36-43 kDa, consistent with the predicted and commonly observed molecular weight range of c-Jun (JUN). c-Jun is a DNA-binding transcription factor and key component of the AP-1 transcriptional complex that regulates gene expression involved in cellular proliferation, differentiation, stress responses, and signal transduction. Detection of c-Jun across multiple human cell lines supports its widespread role in transcriptional regulation and cellular signaling pathways. The observed molecular weight variation is consistent with known post-translational modification and phosphorylation states of c-Jun.

c-Jun Antibody HeLa Cell FACS. Flow cytometry analysis of human HeLa cells using c-Jun Antibody demonstrates a clear rightward shift of the positive cell population (blue) relative to the isotype control (green), consistent with expression of c-Jun (JUN). c-Jun is a DNA-binding transcription factor and key component of the AP-1 signaling complex that regulates gene expression involved in cellular proliferation, differentiation, stress responses, and signal transduction. As a transcriptional regulation protein, c-Jun functions within signaling networks that control cellular adaptation to developmental and environmental stimuli. Cells were blocked with goat serum prior to staining. Red=cells alone; Green=isotype control; Blue=c-Jun antibody.