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banner Search results for Rb1

Phospho-Retinoblastoma Antibody / Rb1 (pS811)

Catalog No : F48447
Applications : DB, IHC, IF, ELISA
Reactivity : Human
Pred. Reactivity :Mouse, Rat
Format : Antigen affinity purified
Confocal immunofluorescent analysis of p-Rb1 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Phospho-Retinoblastoma Antibody / Rb1 (pS608)

Catalog No : F48443
Applications : DB, IHC, ELISA
Reactivity : Human
Format : Antigen affinity purified
Dot blot analysis of phospho-Rb antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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Phospho-Retinoblastoma Antibody / Rb1 (pS780)

Catalog No : F48445
Applications : DB, IHC, ELISA
Reactivity : Human
Pred. Reactivity :Mouse, Rat
Format : Antigen affinity purified
Dot blot analysis of phospho-Rb antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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Phospho-Retinoblastoma Antibody / Rb1 (pS788)

Catalog No : F48446
Applications : DB, IHC, ELISA
Reactivity : Human
Format : Antigen affinity purified
Dot blot analysis of phospho-Rb antibody. 50ng of nonphos-peptide or phos-peptide were adsorbed on their respective dots.
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RB1CC1 Antibody / RB1-inducible coiled-coil protein 1

Catalog No : FY12607
Applications : WB, IHC, IF, ICC/IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of RB1CC1 using anti-RB1CC1 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RB1CC1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Western blot probed with anti-RB1CC1 (FIP200) shows a strong band at ~230 kDa, higher than the predicted ~183 kDa, consistent with the phosphorylated, slow-migrating form of FIP200 commonly observed on SDS-PAGE.
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Retinoblastoma Antibody / Rb1 (clone RB1/2313R)

Catalog No : V3578
Applications : IHC-P
Reactivity : Human, Mouse
Format : Purified
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IHC testing of recombinant RB1 antibody and FFPE human colon carcinoma (clone RB1/2313R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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Retinoblastoma Antibody / Rb1 (clone rRB1/8060)

Catalog No : V4326
Applications : WB, IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human colon carcinoma tissue with recombinant Retinoblastoma antibody (clone rRB1/8060). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Retinoblastoma Antibody / Rb1 (clone RB1/7080R)

Catalog No : V4327
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human bladder carcinoma with recombinant Retinoblastoma antibody (clone RB1/7080R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Retinoblastoma Antibody / Rb1

Catalog No : FY12555
Applications : WB, IHC, ICC/IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of FFPE human A549 cells with RB1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
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Retinoblastoma Antibody / Rb1

Catalog No : F54327
Applications : IHC-P, WB
Reactivity : Human, Mouse, Rat
Format : Purified
IHC testing of FFPE human retina with Retinoblastoma antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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