- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Phospho-RB1 Ser780 Antibody / RB1 Phospho Ser780 Polyclonal Antibody (F48445)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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F48445-0.4ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.4 ml | 439 | |
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F48445-0.08ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.08 ml | 205 |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity |
| UniProt | P06400 |
| Applications | 1:500 1:50-1:100 |
| Limitations | This Phospho-RB1 Ser780 Antibody / RB1 Phospho Ser780 Polyclonal Antibody is available for research use only. |
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Phospho-RB1 Ser780 Antibody / RB1 Phospho Ser780 Polyclonal Antibody recognizes retinoblastoma protein phosphorylated at serine 780, a regulatory site associated with cyclin-dependent kinase signaling and cell cycle progression. Retinoblastoma protein, encoded by the RB1 gene, is a tumor suppressor that regulates the transition from G1 to S phase by controlling the activity of E2F transcription factors. Its growth-suppressive functions are influenced by phosphorylation at multiple residues, which can modify protein interactions and alter the transcriptional programs governing cellular proliferation.
Ser780 is located in the C-terminal region of RB1 and is a well-characterized phosphorylation site associated with cyclin D-dependent CDK4/6 activity. Phosphorylation at this residue is frequently examined as a marker of signaling through the cyclin D-CDK4/6-RB1 pathway. During early G1, mitogenic signals promote cyclin D expression and CDK4/6 activation, contributing to changes in RB1 phosphorylation. Subsequent phosphorylation events involving additional residues and cyclin-dependent kinases help coordinate progression toward DNA replication. However, phosphorylation at Ser780 alone does not establish complete RB1 inactivation or unrestricted E2F activity.
The regulatory significance of Ser780 phosphorylation extends to studies of CDK4/6 inhibition. Pharmacological inhibitors of CDK4/6 can reduce phosphorylation at this site in responsive cells, providing a biochemical indicator of pathway modulation. Interpretation nevertheless requires consideration of total RB1 expression, additional phosphorylation sites, and the cellular context. RB1 phosphorylation is dynamic, and individual residues may respond differently to kinase activity, phosphatase regulation, and cell cycle conditions. Site-specific measurements therefore provide information that cannot be obtained from total protein abundance alone.
Disruption of the cyclin D-CDK4/6-RB1 pathway contributes to abnormal proliferation in numerous cancers. Increased cyclin D expression, CDK4/6 activation, or loss of CDK inhibitors can alter RB1 phosphorylation and weaken normal cell cycle restrictions. Conversely, tumors lacking functional RB1 may respond differently to therapies targeting upstream cyclin-dependent kinases. Distinguishing phosphorylation-dependent regulation from RB1 protein loss is consequently important when examining mechanisms of tumor growth and therapeutic response. This Phospho-RB1 Ser780 Antibody provides a site-specific reagent for investigating RB1 phosphorylation, CDK4/6 pathway activity, and the molecular regulation of G1 progression.
Explore our Retinoblastoma Antibody FCD-18, a rabbit monoclonal antibody targeting RB1, for an additional option with Western blot, immunohistochemistry, and immunofluorescence validation.
Titration of the Phospho-RB1 Ser780 Antibody / RB1 Phospho Ser780 Polyclonal Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This Phospho-RB1 Ser780 Antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS780 of human Rb.
Aliquot the Phospho-RB1 Ser780 Antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Phospho-Rb Ser780 antibody, Phospho-Retinoblastoma Ser780 antibody, Phospho-pRb Ser780 antibody, Phosphorylated RB1 Ser780 antibody, Retinoblastoma Protein Phospho Ser780 antibody
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