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banner Search results for Pleckstrin

RAPH1 Antibody / Ras-associated and pleckstrin homology domains-containing protein 1

Catalog No : FY12411
Applications : WB, IHC, ELISA
Reactivity : Human, Mouse
Format : Lyophilized
Immunohistochemical staining of RAPH1 using anti-RAPH1 antibody. RAPH1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAPH1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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PLEKHB1 Antibody / Pleckstrin homology domain-containing family B member 1

Catalog No : FY13370
Applications : WB, ELISA
Reactivity : Human, Monkey, Mouse, Rat
Format : Lyophilized
Western blot analysis of PLEKHB1 using anti-PLEKHB1 antibody. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: monkey RF/6A whole cell lysates, Lane 5: rat eye tissue lysates, Lane 6: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHB1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for PLEKHB1 at approximately 27 kDa. The expected molecular weight of PLEKHB1 is ~27 kDa.
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PHLDA2 Antibody / Pleckstrin homology-like domain, family A, member 2

Catalog No : R33928
Applications : IHC-P, WB, IF, FACS, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
IHC testing of FFPE human placental tissue with PHLDA2 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
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PHLDB3 Antibody / Pleckstrin homology-like domain family B member 3

Catalog No : FY12062
Applications : WB, IHC, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
IHC analysis of PHLDB3 using anti-PHLDB3 antibody. PHLDB3 was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PHLDB3 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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PLEKHG5 Antibody / Pleckstrin homology domain-containing family G member 5

Catalog No : RQ8010
Applications : WB, IHC-P, IF, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human intestinal cancer tissue with PLEKHG5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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PLEK Antibody / Pleckstrin

Catalog No : RQ7023
Applications : WB, IHC-P, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
IHC staining of FFPE human tonsil tissue with PLEK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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PLEK Antibody / Pleckstrin

Catalog No : R34045
Applications : WB, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of human PBMC lysate with Pleckstrin antibody at 0.05ug/ml. Predicted molecular weight: ~40kDa, observed here at ~45kDa.
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PLEK Antibody / Pleckstrin (clone 28P96)

Catalog No : RQ8727
Applications : WB
Reactivity : Human
Format : Purified
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Western blot testing of human Raji cell lysate with PLEK antibody. Predicted molecular weight ~40 kDa.
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Kindlin-2 Antibody / KIND2 / FERMT2

Catalog No : RQ7522
Applications : WB, IHC-P, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
IHC staining of FFPE human spleen tissue with FERMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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PLEKHA1 Antibody / TAPP-1

Catalog No : RQ8032
Applications : WB, IHC-P, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human lung cancer tissue with TAPP-1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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