• Tel: 858.663.9055
  • separatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner

Loading Control Antibody / Antibodies

Home >> Antibodies >> Loading Control Antibody / Antibodies

Loading Control Antibody / Antibodies

Are you familiar with the concept of loading control antibodies in western blot analysis? If not, you may be missing out on a crucial step in ensuring the accuracy and reliability of your protein analysis results.

When conducting western blot experiments, researchers must carefully control for variations in sample loading, transfer efficiency, and protein expression levels. This is where loading control antibodies come into play. These specialized antibodies target proteins that are constitutively expressed at relatively constant levels in cells, serving as internal references for normalization and comparison of protein expression levels.

One of the key advantages of using loading control antibodies in western blotting is their ability to account for potential discrepancies in protein loading across samples. By including a loading control antibody alongside your target protein of interest, you can adjust for variations in sample preparation, gel loading, and transfer efficiency, ensuring accurate and reliable results.

In addition to improving the accuracy of protein quantification, loading control antibodies also help to validate the specificity and integrity of your western blot experiments. By confirming that the loading control antibody recognizes a single, specific band at the expected molecular weight, researchers can confidently interpret their results and draw meaningful conclusions about protein expression levels.

In conclusion, loading control antibodies play a crucial role in western blot analysis by providing internal references for normalization, ensuring accurate protein quantification, and validating the specificity of experimental results. By incorporating loading control antibodies into your experimental workflow, you can enhance the reliability and reproducibility of your research findings, ultimately leading to more robust and impactful scientific discoveries. 
 
Found all antibodies, displaying 1 to 10
GAPDH Antibody (clone GAP259)
Catalog No : N1074
Applications : WB, IHC-P
Reactivity : Human, Mouse, Rat, Primate, Dog
Format : Purified
Beta Actin Antibody
Catalog No : N1037
Applications : WB
Reactivity : Human, Mouse, Rat, Monkey, Rabbit, Chicken, Dog
Format : Purified
Beta Tubulin Antibody / TUBB2A
Catalog No : N1038
Applications : WB
Reactivity : Human, Mouse, Rat, Primate, Dog
Format : Purified
Actin Antibody (clone AC-40)
Catalog No : R30001
Applications : WB, IHC-F
Reactivity : Human, Mouse, Rat, Chicken
Format : Ascites
Beta Actin Antibody (clone AC-15)
Catalog No : R30100
Applications : WB, IHC-P
Reactivity : Human, Mouse, Rat
Format : Ascites
Beta Actin Antibody
Catalog No : R30971
Applications : WB
Reactivity : Human, Mouse, Rat, Monkey, Zebrafish, Chicken
Format : Antigen affinity purified
Alpha Tubulin Antibody / TUBA1A (clone DM1A)
Catalog No : R30095
Applications : WB, IHC-P
Reactivity : Human, Mouse, Rat, Chicken
Format : Ascites
Beta Tubulin Antibody / TUBB1 (clone TUB 2.1)
Catalog No : R30098
Applications : WB, IHC-P
Reactivity : Human, Mouse, Rat
Format : Ascites
Alpha Actin Antibody / ACTA2 (smooth muscle) (clone 1A4)
Catalog No : R30094
Applications : WB, IHC-P, IHC-F, IF
Reactivity : Human, Mouse, Rat
Format : Ascites
TBP Antibody / TATA Binding Protein
Catalog No : R30610
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified